Biosensor Specifications

Download a PDF version of the Biosensor Specifications Sheet here.

WPI offers a range of biosensors for monitoring nitric oxide, hydrogen peroxide, oxygen and hydrogen sulfide. Specifications for these sensors are detailed below.

  • The macrosensors are 2mm "wet" sensors. These sensors are installed in a metal "sleeve" that looks like a tiny soda straw.The sleeve has a gas permeable membrane at the tip, and it is filled with an electrolyte. When it is immersed in a solution, the gas in solution (for example, nitric oxide) diffuses through the membrane, and the sensor measures it.
  • The microsensors are "dry" sensors. Most microsensors monitor nitric oxide, and there is also a hydrogen peroxide microsensor available.

Macrosensors (2mm)

Macrosensors (2mm)

WPI's 2mm macrosensors are uniquely designed with an internal reference electrode. These sensors are designed for use with the TBR1025, or TBR4100. The ISO-OXY-2 and the OXELP sensors have the same specifications. However, they have different connectors, because the OXELP is designed for use with the ISO2 dissolved oxygen meter.

NOTE: The sensitivity of WPI sensors varies with length and diameter. Each sensor is unique and will come with its own Sensor Performance Evaluation sheet. WPI sensors typically conform to the specifications offered in this article. Custom sensors, of course, will have unique tolerances and sensitivities.

  ISO-NOP ISO-HPO-2 ISO-OXY-2
OXELP
ISO-H2S-2
Species Nitric Oxide Hydrogen peroxide Oxygen Hydrogen Sulfide
Outside Diameter 2mm 2mm 2mm 2mm
Response Time < 5 sec. <5 sec. <10 sec. <5 sec.
Lowest Detection Limit/Range 1nM <100nM 0.1% <5nM
Upper Detection Limit/Range 80µM (beyond 80µM, sensor accuracy is not guaranteed) 100µM 100% 100µM
Nominal Sensitivity-New Sensor ≤2pA/nM 8pA/μM 0.3-0.6nA/% 2pA/nM
Interferent (Selectivity Coefficient) NaNO2 (10-6 or better)      
Baseline Drift <1pA/min 0.1pA/min. <1%/min. 0.1pA/min.
Poise Voltage 865mV 400mV -700mV 150mV
Typical Quiescent Baseline Current, 25ºC 3,000pA 850pA 15-40nA 15nA
Acceptable Baseline Range 1,000-8,000 pA 20-4,000pA 15-40nA 2.5-75nA
Typical Polarization Time 12+ hrs 2+ hours 1+ hours, 95% 12+ hours
Recommended Polarization Solution 0.1M KI/H2SO4 0.1M PBS 0.1M PBS 0.1M PBS
Temperature Dependent Yes Yes Yes Yes
Physiological Interference None None None None

Microsensors

  NOPF200 NOPF200-LXX NOPF100
NOPF100-L
NOP70-LXX  NOPF500-Cxx NOP3005 NOP3020 NOP30-L NOP007 NOPNM HPO-100 H2S-100
Species Nitric Oxide H202  H2S
Outside Diameter (µm) 200 200 100 70 500  30 30 30 7 Conical tip:100nm
Fiber: 7µm
100 100
Available Length2 (mm) 1-51 1-101 1-51 3 5-10  0.5 2 3 2 2 1-41  1-51
Response Time (sec.) < 5 < 5 < 5 < 3 < 3<10  <3 < 3 < 3 < 3 < 3 < 5  ~5
Lowest Detection Limit/Range (nM) 0.2 0.2 0.2 1 10.2  1 1 1 0.5 0.5 < 10  <5
Nominal Sensitivity-New Sensor2 (pA/nM) ≥20 ≥50 ≥10 ≥10 ≥20  ≥1 ≥1.5 ≥1 ≥1 ≥0.5 ≥1  1-4
Baseline Drift (pA/min) none none none none none  none none none none none <2.0  <2
Poise Voltage (mV) 865 865 865 865 865  865 865 865 865 865 400  150
Typical Quiescent Base-line Current, 25ºC (pA) 2500 3500 2000 3500 5000  1000 500 500 300 300 <1000  1000
Acceptable Baseline Range (pA) 500-8000 500-8000 500-8000 2000-6000 3000-25000  150-3500 500-5000 20-6500 200-1500 50-100 150- 1200  100-2000
Typical Polarization Time (hrs) 2+ 8+ 2+ 2+ 8+  1+ 1+ 1+ 1+ 1+ 1+  4+

1Sensor available in 1mm length increments (for example, 1mm, 2mm, 3mm...).
2Sensor sensitivity varies with length and diameter.

NOPNM

NOPNM 

ISO-NOPF

ISO-NOPF

ISO-NOP007, ISO-NOP30

ISO-NOP007, ISO-NOP30

ISO-HPO-100

ISO-HPO-100

 

See Selection