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Biosensor Specifications
April 29, 2013
Download a PDF version of the Biosensor Specifications Sheet here.
WPI offers a range of biosensors for monitoring nitric oxide, hydrogen peroxide, oxygen and hydrogen sulfide. Specifications for these sensors are detailed below.
- The macrosensors are 2mm "wet" sensors. These sensors are installed in a metal "sleeve" that looks like a tiny soda straw.The sleeve has a gas permeable membrane at the tip, and it is filled with an electrolyte. When it is immersed in a solution, the gas in solution (for example, nitric oxide) diffuses through the membrane, and the sensor measures it.
- The microsensors are "dry" sensors. Most microsensors monitor nitric oxide, and there is also a hydrogen peroxide microsensor available.
Macrosensors (2mm)
WPI's 2mm macrosensors are uniquely designed with an internal reference electrode. These sensors are designed for use with the TBR1025, or TBR4100. The ISO-OXY-2 and the OXELP sensors have the same specifications. However, they have different connectors, because the OXELP is designed for use with the ISO2 dissolved oxygen meter.
NOTE: The sensitivity of WPI sensors varies with length and diameter. Each sensor is unique and will come with its own Sensor Performance Evaluation sheet. WPI sensors typically conform to the specifications offered in this article. Custom sensors, of course, will have unique tolerances and sensitivities.
ISO-NOP | ISO-HPO-2 | ISO-OXY-2 OXELP |
ISO-H2S-2 | |
Species | Nitric Oxide | Hydrogen peroxide | Oxygen | Hydrogen Sulfide |
Outside Diameter | 2mm | 2mm | 2mm | 2mm |
Response Time | < 5 sec. | <5 sec. | <10 sec. | <5 sec. |
Lowest Detection Limit/Range | 1nM | <100nM | 0.1% | <5nM |
Upper Detection Limit/Range | 80µM (beyond 80µM, sensor accuracy is not guaranteed) | 100µM | 100% | 100µM |
Nominal Sensitivity-New Sensor | ≤2pA/nM | 8pA/μM | 0.3-0.6nA/% | 2pA/nM |
Interferent (Selectivity Coefficient) | NaNO2 (10-6 or better) | |||
Baseline Drift | <1pA/min | 0.1pA/min. | <1%/min. | 0.1pA/min. |
Poise Voltage | 865mV | 400mV | -700mV | 150mV |
Typical Quiescent Baseline Current, 25ºC | 3,000pA | 850pA | 15-40nA | 15nA |
Acceptable Baseline Range | 1,000-8,000 pA | 20-4,000pA | 15-40nA | 2.5-75nA |
Typical Polarization Time | 12+ hrs | 2+ hours | 1+ hours, 95% | 12+ hours |
Recommended Polarization Solution | 0.1M KI/H2SO4 | 0.1M PBS | 0.1M PBS | 0.1M PBS |
Temperature Dependent | Yes | Yes | Yes | Yes |
Physiological Interference | None | None | None | None |
Microsensors
NOPF200 | NOPF200-LXX | NOPF100 NOPF100-L |
NOP70-LXX | NOPF500-Cxx | NOP3005 | NOP3020 | NOP30-L | NOP007 | NOPNM | HPO-100 | H2S-100 | |
Species | Nitric Oxide | H202 | H2S | |||||||||
Outside Diameter (µm) | 200 | 200 | 100 | 70 | 500 | 30 | 30 | 30 | 7 | Conical tip:100nm Fiber: 7µm |
100 | 100 |
Available Length2 (mm) | 1-51 | 1-101 | 1-51 | 3 | 5-10 | 0.5 | 2 | 3 | 2 | 2 | 1-41 | 1-51 |
Response Time (sec.) | < 5 | < 5 | < 5 | < 3 | < 3<10 | <3 | < 3 | < 3 | < 3 | < 3 | < 5 | ~5 |
Lowest Detection Limit/Range (nM) | 0.2 | 0.2 | 0.2 | 1 | 10.2 | 1 | 1 | 1 | 0.5 | 0.5 | < 10 | <5 |
Nominal Sensitivity-New Sensor2 (pA/nM) | ≥20 | ≥50 | ≥10 | ≥10 | ≥20 | ≥1 | ≥1.5 | ≥1 | ≥1 | ≥0.5 | ≥1 | 1-4 |
Baseline Drift (pA/min) | none | none | none | none | none | none | none | none | none | none | <2.0 | <2 |
Poise Voltage (mV) | 865 | 865 | 865 | 865 | 865 | 865 | 865 | 865 | 865 | 865 | 400 | 150 |
Typical Quiescent Base-line Current, 25ºC (pA) | 2500 | 3500 | 2000 | 3500 | 5000 | 1000 | 500 | 500 | 300 | 300 | <1000 | 1000 |
Acceptable Baseline Range (pA) | 500-8000 | 500-8000 | 500-8000 | 2000-6000 | 3000-25000 | 150-3500 | 500-5000 | 20-6500 | 200-1500 | 50-100 | 150- 1200 | 100-2000 |
Typical Polarization Time (hrs) | 2+ | 8+ | 2+ | 2+ | 8+ | 1+ | 1+ | 1+ | 1+ | 1+ | 1+ | 4+ |
1Sensor available in 1mm length increments (for example, 1mm, 2mm, 3mm...).
2Sensor sensitivity varies with length and diameter.
ISO-NOPF
ISO-NOP007, ISO-NOP30
ISO-HPO-100
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