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Product Information
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August 23, 2013
Researchers at the University of Michigan are using WPI's PV820 for injecting a morpholino solution into the lumen of the otic vesicle of 1-day old zebrafish embryos. Then, they use electroporation to introduce mif and mif-like morpholinos into the developing inner ear tissues.
Check out the ...more
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August 20, 2013
The EndOhm series of chambers, when used with WPI’s EVOM2 resistance meter or the Millicell ERS, are designed to provide reproducible and accurate resistance measurements of endothelial tissue in culture cups. Resistance values obtained with the EndOhm are consistent with those obtained using a well-designed Ussing Chamber. If an EndOhm is properly cared for, it will enjoy a much longer life. In this article, we will examine how often and what method should be used for cleaning an EndOhm electrode.
How Do I Clean the EndOhm?
With use, the electrode surface can become...more
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May 22, 2013
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May 02, 2013
Microinjection uses either a metal needle or a glass micropipette to inject small liquid volumes. For example, genetic material may be inserted into a living cell, a drug introduced into an eye or brain, or fluid injected into a muscle. Typically, microinjection is performed under a microscope. A stereotaxic setup may be required. WPI offers a variety of pumps along with special syringes, stereotaxic frames, glass capillaries and needles. Here we will highlight our microinjection pumps. The setup you choose depends on the size of injection aliquots, the volume to be injected and the size of needle or glass tip chosen. The products described below are listed in order by the smallest volume they can inject. See the comparison chart below or the related post links at the bottom.
Pressure Injectors
WPI's new generation of pressure microinjectors are quickly replacing the very popular legacy line of pnuematic valve...more
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May 01, 2013
Surgical Loupes help to alleviate eye strain by enlarging the image when you are working on tiny subjects or conducting precision operations. They are portable and easier to use than a surgical microscope. However, they are not created equal, and choosing the pair that's right for you is important to your satisfaction.
Factors Involved in Choosing Loupes
Choosing the correct surgical loupes for your application involves several factors, including resolution, working distance, field of view, depth of field, magnific
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May 01, 2013
The barbed tubing assortment kit contains over 250 items. All the items are pictured on a grid below. The darker lines in the image grid represent centimeters, and the smaller ones are 2.5 mm apart.
...moreLuer to Tubing Coupler Assortment Kit P/N 504955 (Nylon)
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May 01, 2013
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May 01, 2013LabScribe is the powerful software included with Lab-Trax™ (formerly called DataTrax). The acquisition and data display in LabScribe is easy and straight forward. It supports real units and a time-based display that is not coupled to sample rate. You can display up to 1,000,000 data points per channel on the screen. Scrolling, zoom-in and zoom-out tools, together with a searchable list of user interventions, make finding important areas of data easy. And, of course, you can print or export anything that you see.
The Main Display
Data recorded with LabScribe is presented as distance-per-unit of time (for example, cm/sec) exactly as it would be on a traditional paper chart recorder. The x-axis display is compressed or expanded using point and click icons in the LabScribe toolbar. LabScribe ’s display can smoothly scroll data at any speed. The display...more
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May 01, 2013
Order code Description DMF1000 Description: Complete Microforge System; includes Programmable Digital Controller and Microscope Application: Fabrication of special shapes of glass micropipettes. -
May 01, 2013
Stretch receptors are specialized fibers that are in parallel to the fibers in muscle. The receptors stretch as the muscle is lengthened, and they generate action potentials. The frequency of these action potentials is proportional to the length of muscle stretch and the position of the muscle. Through sensory nerve fibers from the stretch receptors, the action potentials and their frequency provide feedback to the central nervous system that will modulate reflex responses and motor control of the muscle.
Setup
For these studies, the whole muscle and the nerve that innervates it are isolated from the organism. A muscle and its nerve, like the Soleus, is isolated and placed in the cuvette of an SI-MT or SI-HTB muscle research system. The muscle is positioned so that the myoneural junction (the place where the nerve innervates the muscle) is on the top side of the muscle. The ends of...more
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May 01, 2013
Abstract
Concentrations of DNA in solution (31µg/mL and 688µg/mL) were measured with a spectrometer and UV/VIS light source in a DIPUV-Mini. Due to the 2mm pathlength, use of a DIPUV-Mini does not require a pre-measurement dilution within this concentration range, thus a potential source of error was eliminated.
Experimental Procedure...more
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May 01, 2013
Abstract
Concentrations of DNA in solution (31µg/mL and 561µg/mL) were measured with a spectrometer and UV/Vis light source in a cuvette. A 2mm pathlength
cuvette does not require a pre-measurement dilution within this concentration range, thus a potential source of error was eliminated. Although a 2mm cuvette has a total internal volume of 0.7mL...more -
May 01, 2013
Cuvettes come in a variety of shapes and sizes, but one of the most important specifications of a cuvette is its Z-dimension. The Z-dimension of an instrument (cuvette holder or spectrometer) is the distance from the bottom of the cuvette chamber floor to the center of its light beam (see image). A cuvette’s Z-dimension must match the Z-dimension of the instrument with which it will be used. Each manufacturer designs its instruments with a specific Z-dimension. Common Z-dimensions include 8.5 and 15mm, and sometimes 20mm. When purchasing small volume cuvettes, the correct Z-dimension becomes critical. Matching the Z-dimension of the cuvette to the Z-dimension of the instrument ensures that the light beam passes through the center of small samples.The table below shows the standard Z-dimension of the spectrometer sample compartments for many manufacturers.
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May 01, 2013
The Nanoliter injector is a micro-processor controlled injection system that uses direct piston displacement. The collet assembly (exploded view) is shown in the image below. The glass pipette fits into the white, plastic sleeve (spacer). When the collet is screwed in place securely, the black rubber washers are compressed and hold the glass firmly in place.
Nanoliter 2000
A side view of the assembled Nanoliter injector is shown below.
Nanoliter 2010
As the plunger is depressed, it pushes into the glass to expel the liquid in the pipette. Eventually, the diameter of the plunger matches that...more
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April 30, 2013
Suction electrodes are used with muscle research systems to record the action potentials from the stretch receptors at the same time that the length and tension of the muscle are recorded.
Materials
- soldering iron with solder
- wire strippers
- wooden-handled dissecting pin
- alcohol burner
- can or tube of contact or plastic cement
- fine flat file
- emery cloth
- electrical tape
- popsicle stick
- two pieces of chlorided silver wire (0.005” dia, 5” long)
(See Chloriding Silver Wire.) - three feet of shielded, two-conductor, insulated cable
- three color-codedconnectors that will mate to the connectors...more