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Microinjection
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April 27, 2021
The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here is how to run a program that will pull two identical microelectrodes.
Loading a Program
We will use the keypad to navigate through the display.
- Use the arrows to navigate to LOAD (00). The top and bottom arrows on the keypad navigate numbers in increments of 1. The left and right arrows on the keypad navigate numbers in increments of 10. Press ENTER. The number will start blinking.
- Use the arrows to navigate to the program number you would like load. Once you reach the desired program, press ENTER...more
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April 23, 2021
The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here is a quick overview to get you started quickly.
Quick Start Setup
- Plug the power cord into the wall outlet. An LED light on the power supply illuminated when the unit is connected to power.
- Plug the power cord into the rear panel of the PUL-1000. Then, the power switch on the side panel illuminates.
- Press the Power button to turn on the display.
- To open the glass capillary clamps, place your thumb under the clamp and depress the release button with your index finger. This allows the carriages to move freely.
- Push the carriages together toward the center.
- Install the silicone glass capillary...more
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April 22, 2021
The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will see how to load a piece of glass into the carriage.
The glass capillary is held by clamps mounted on two movable carriages. Both carriages synchronously slide as a program is executed.
- Manually slide the carriages together when you are loading the glass.
- To open the clamps, place your thumb under the clamp and depress the release button with your index finger.
- Slide the glass capillary into the groove from one side. Slide it through the center of heating filament onto the...more
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April 22, 2021
The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here are the resolutions for some common issues with the puller.
Puller Stops
If the puller stops in the middle of a pull and the unit beeps, one of two issues may be the culprit.
- The unit may be over heating. Allow the unit time to cool down before attempting to pull more glass.
- If a program fails in the middle of a pull, you may have exceeded the parameters of the unit. For example, when you add up the distance of travel for all the stages, you may have exceeded the maximum range of travel. Check your program. You may need to alter the heating or travel parameters to conform with the unit’s maximums.
Glass...more
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April 22, 2021
The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. We will show you how to run a glass softening test.
You should run the Softening Test when:
- You change the filament
- Lot numbers or capillary types change
- You create or modify a program
- The ambient environment changes
- Press the STOP key to quit any running program. The following window displays.
- Mount a glass capillary on the carriage.
- Press the START key to run the Glass Capillary Softening...more
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April 09, 2021
Let’s look at the PUL-1000, a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will examine the design of the unit.
Tempered Glass Lid
For safety, the lid should be closed whenever you are pulling glass or moving the unit.
Capillary Glass Clamp Pads
These two silicone clamp pads securely hold the glass when you are pulling pipettes. Check the glass OD and install appropriate pads accordingly.
Heating Filament
The PUL-1000 comes with a circular platinum/iridium box filament, which may be easily replaced...more
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February 02, 2021
WPI pre-pulled pipettes (MicroTips) are a popular choice for pharmaceutical companies carrying out CCIT (Closed Container Integrity Testing). Each MicroTip is individually tested and inspected and can be shipped with a certificate of compliance.
CCIT is an important part in understanding the biological safety and...more
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January 29, 2021
The system depicted includes components often favored by researchers:
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January 28, 2021
World Precision Instruments (WPI) offers a broad range of research instruments for zebrafish (Danio Rerio) research, from the time the genetics of the subject are modified through the point where the physiological effects of the change are monitored. Whether researchers are performing microinjection or making cardiovascular measurements, the WPI product line includes instruments for research on oocytes, embryos, larvae and adult zebrafish.
Enhanced Microinjection Efficiency for Genetic Modification
The customizable Zebrafish Microinjection Toolbox includes...more
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September 08, 2020
[by Gabe Gonzalez]
The PV850 Injector is designed to simplify intracellular injection and a variety of other microinjection tasks. The PV850 uses regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection maximum of 87 PSI. The PV850 Microinjector offers separate regulated compensation (back filling prevention) and ejection pressures with a precision timing circuit that switches from injection pressure to compensation pressure automatically. Timing, injection pressure and compensation pressure are adjusted independently using the intuitive touch-screen user interface. Time intervals can range from 2 seconds down to 10 ms or less, depending on the injection pressure setting...more
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September 08, 2020
[by Gabe Gonzalez]
The PV850 Injector is designed to simplify intracellular injection and a variety of other microinjection tasks. The PV850 uses regulated air pressure for injecting cells with fluid. Injected volumes range from picoliters to nanoliters. The port supplies positive pressure for high-pressure ejection maximum of 8 7PSI. The PV850 Microinjector offers separate regulated compensation (back filling prevention) and ejection pressures with a precision timing circuit that switches from injection pressure to compensation pressure automatically. Timing, injection pressure and compensation pressure are adjusted independently using the intuitive touch-screen user interface. Time intervals can range from 2 seconds down to 10 ms or less, depending on the injection pressure setting...more
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September 03, 2020
Designed to simplify intracellular injection and a variety of other microinjection tasks, WPI’s PicoPumps (PV830 and PV820) use precisely regulated pressures for securing cells and injecting them with fluid. Injected volumes range from picoliters to nanoliters. Separate ports supply positive and negative pressure—positive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. In this quick series, you can see how to setup a PicoPump.
1. What's Included with a PV Pump
In this first video (#1) you can see all the components of the system as they unpack the system.
...more
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July 13, 2020
[by Alec Dickson]
Easy to Configure Using the SmartTouch Interface
The UMP3 UltraMicroPump is easy to configure using the SMARTouch interface. It is pre-programmed for popular microsyringes and allows for user-defined syringe parameters, too. This ensures nearly universal compatibility. You can control up to two pumps, either simultaneously or independently, and the optional foot switch means hands-free operation. You can instantly verify actual injection volume on the graphical display. You can even use the touch screen while wearing gloves.
Ensure Reliable Injections of Small Volumes
The extremely fine thread-pitch of the UMP3 drive shaft ensures reliable injections of small volumes. When you are using a 10-microliter syringe...more
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June 16, 2020
[by Alec Dickson]
WPI's FluoroDish™ tissue culture dishes provide exceptional imaging quality for many applications requiring the use of inverted microscopes such as high-resolution image analysis, microinjection and electrophysical recording of fluorescent-tagged cells. We have a 50 mm diameter dish and two types of 35 mm diameter dishes.
Better Optical Properties than PolycarbonateEach WPI dish has a flat (0.17mm±0.01mm thick), optical quality glass bottom, allowing...more
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May 15, 2020
The UltraMicroPump3 is a versatile microinjection syringe pump designed to support the needs of a busy lab. The UMP3 Microinjector is suitable for delivery of media from nanoliters to milliliters. the UMP3 injecotor’s user interface and system design enables delivery of highly accurate and repeatable injections. The choice for thousands of scientists, it is widely cited in research papers.
Intuitive Touchscreen Display for Easy SetupThe SMARTouch controller has an intuitive touch screen interface and an easy-to-use menu for quick configuration of the syringe size, volumes and flow...more