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Microinjection Videos
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August 05, 2022
The ATC2000 is a low noise heating system for maintaining animal body temperature during expirimental procedures. Here we show you how to use the adaptive mode on the ATC2000 system.
- Press the Config button to access the configuration menu. Press the Config button again until the adaptive mode Enable displays.
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April 28, 2022
The ATC2000 is a low noise heating system for maintaining animal body temperature during experimental procedures. The ATC2000 uses a digital PID controller to regulate the application of power to the heating plate to achieve the desired temperature at the monitored sensor. PID stands for Proportional, Integral and Differential. Here’s how PID control works:
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January 17, 2022
The DMF1000 is a microprocessor-controlled microforge designed for fabrication of both small patch clamp glass pipettes and larger injection pipettes. The DMF1000 can be used for polishing patch clamp pipettes, microforging holding pipettes, microforging beveled injection pipettes, and for calibrating pipette tips for microinjection. Here we will show you how to install the microforge filament on your microscope.
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January 17, 2022
The DMF1000 is a microprocessor-controlled microforge designed for fabrication of both small patch clamp glass pipettes and larger injection pipettes. The DMF1000 can be used for polishing patch clamp pipettes, microforging holding pipettes, microforging beveled injection pipettes, and for calibrating pipette tips for microinjection. Here we will show you how to configure the DMF1000 controller.
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July 23, 2021
Do you want to take the next step in your CRISPR-CAS9 projects, making the injection of biomolecules in your cell lines more efficient? You can now increase the viability of injected embryos with WPI’s MICRO-ePORE, which offers improved repeatability and functionality for pinpoint cell penetration for targeted microinjections in transgenesis.
Improve Embryo Survival with Pinpoint Cell Penetration
Principle of MICRO-ePORE™ Technology
- Classic Microinjection of biomolecules with capillaries causes permanent damage to the cell membrane...more
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May 27, 2021
This video shows how to setup the heavy boom microscope stand (502004), which can be used with a PZMIII or PZMIV microscope head. The PZMIII and PZMIV require the 502009 76mm focus mount. The setup of the 505606 heavy boom stand is identical. The 505606 uses the 505610 focus mount that is designed for use with the Enhanced Reality Macroscope. The focus mounts are sold separately.
Stand Components
These are the pieces of the boom stand.
(1) Heavy Base
(1) Vertical Post
(1) Guard Ring
(1) Horizontal Bar
(1) Cross Block
(1) Anchoring Screw with Locking Washer
(1) Hex Key
(1) Clutch Handle...more
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May 27, 2021
This video shows how to setup the articulating arm clamp stand for a microscope (502007), which can be used with a PZMIII microscope head. The PZMIII requires the 502009 76mm focus mount. The setup of the 505607 articulating arm stand is identical. The 505607 uses the 505610 focus mount that is designed for use with the Enhanced Reality Macroscope. The focus mounts are sold separately.
Stand Components
These are the pieces of the stand.
(1) Table clamp base
(1) Middle horizontal bar
(1) Adjustable forearm
(2) Clutch handles
(2) Adjustment screws
(2) Ring washers (white)
Set...more
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May 27, 2021
This video shows how to setup the post stand for a microscope (502007), which can be used with a PZMIII microscope head. The PZMIII or PZMIV require the 504947 76mm focus mount. The setup of the 505608 post stand is identical. The 505608 uses the 505609 focus mount that is designed for use with the Enhanced Reality Macroscope. The focus mounts are sold separately.
Stand Components
These are the pieces of the stand.
(1) Base
(1) Vertical Post
(1) Vertical Post Extension
(1) Guard Ring Support
(1) Reversible Plate
(1) Clutch Handle
(1) Post Cap
(1) Focus Mount
504947 Focus Mount included with the 503102...more
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April 27, 2021
The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here is how to run a program that will pull two identical microelectrodes.
Loading a Program
We will use the keypad to navigate through the display.
- Use the arrows to navigate to LOAD (00). The top and bottom arrows on the keypad navigate numbers in increments of 1. The left and right arrows on the keypad navigate numbers in increments of 10. Press ENTER. The number will start blinking.
- Use the arrows to navigate to the program number you would like load. Once you reach the desired program, press ENTER...more
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April 23, 2021
The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here is a quick overview to get you started quickly.
Quick Start Setup
- Plug the power cord into the wall outlet. An LED light on the power supply illuminated when the unit is connected to power.
- Plug the power cord into the rear panel of the PUL-1000. Then, the power switch on the side panel illuminates.
- Press the Power button to turn on the display.
- To open the glass capillary clamps, place your thumb under the clamp and depress the release button with your index finger. This allows the carriages to move freely.
- Push the carriages together toward the center.
- Install the silicone glass capillary...more
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April 22, 2021
The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will see how to load a piece of glass into the carriage.
The glass capillary is held by clamps mounted on two movable carriages. Both carriages synchronously slide as a program is executed.
- Manually slide the carriages together when you are loading the glass.
- To open the clamps, place your thumb under the clamp and depress the release button with your index finger.
- Slide the glass capillary into the groove from one side. Slide it through the center of heating filament onto the...more
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April 22, 2021
The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here are the resolutions for some common issues with the puller.
Puller Stops
If the puller stops in the middle of a pull and the unit beeps, one of two issues may be the culprit.
- The unit may be over heating. Allow the unit time to cool down before attempting to pull more glass.
- If a program fails in the middle of a pull, you may have exceeded the parameters of the unit. For example, when you add up the distance of travel for all the stages, you may have exceeded the maximum range of travel. Check your program. You may need to alter the heating or travel parameters to conform with the unit’s maximums.
Glass...more
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April 22, 2021
The PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. We will show you how to run a glass softening test.
You should run the Softening Test when:
- You change the filament
- Lot numbers or capillary types change
- You create or modify a program
- The ambient environment changes
- Press the STOP key to quit any running program. The following window displays.
- Mount a glass capillary on the carriage.
- Press the START key to run the Glass Capillary Softening...more
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April 09, 2021
Let’s look at the PUL-1000, a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes. Here we will examine the design of the unit.
Tempered Glass Lid
For safety, the lid should be closed whenever you are pulling glass or moving the unit.
Capillary Glass Clamp Pads
These two silicone clamp pads securely hold the glass when you are pulling pipettes. Check the glass OD and install appropriate pads accordingly.
Heating Filament
The PUL-1000 comes with a circular platinum/iridium box filament, which may be easily replaced...more
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February 25, 2021
NanoFil™ is a unique low volume syringe developed for improved microinjection in mice and other small animals. RPE and IO kits are specially designed for “hands-free” and oil-free sub-microliter injection. Originally developed for retinal pigment epithelium and intraocular injection, they can also be used for other applications.
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