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EVOM2

  1. How To Select Electrodes For Making TEER Measurements
    November 28, 2018
    Selection of Electrodes for TEER Measurements Transepithelial electrical resistance (TEER), also referred as the transepithelial resistance (TER) is used to monitor cellular health. TEER is comprised of measurements of the transcellular pathway (i.e., resistance due to an individual cell) and paracellular pathway (i.e., resistance due to the formation of the cellular junctions). TEER is commonly used to monitor cellular confluence. TEER values can indicate changes in the cellular monolayer permeability, showing the monolayer barrier function of cells such as, endothelial (brain microvessel) and epithelial (alveolar, kidney, and intestinal) cells. High TEER values generally reflect tighter cellular monolayers or cellular junctions (Lewis 1996, Matter and Balda 2003, Denker and Sabath 2011). A few major benefits of WPI TEER measurement systems are described below. The TEER values (electrophysiological analysis) can be combined with other analysis methods to further understand
  2. FAQs about TEER Measurement
    February 02, 2015
    Here are some frequently asked questions (FAQ) about TEER measurement using an EVOM2. Are the electrical resistance and transepithelial electrical resistance (TEER) the same thing? What is an EndOhm chamber? How is an EVOM2 used for measuring confluence? Why use an EndOhm instead of a STX? How do I clean my electrode? What about electrode preconditioning? Can you give me a simple data acquisition system for TEER? What are the TEER measurement challenges that I may encounter? Can you suggest some experimental parameters that can be controlled to obtain consistent TEER results?
  3. Recording TEER Measurements from an EVOM2
    November 11, 2014
    The EVOM2 is the classic, hand-held instrument for making Trans Epithelial Electrical Resistance (TEER) measurements. The REMS system adds a robot and data recording for automated TEER measurements. There may be times when you don't have a robot, but would still like to have the data recording capabilities of the REMS system. With a little ingenuity, you can do just that. Here, we will show you how you can add data recording to your standard EVOM2
  4. FAQ: Troubleshooting Unstable Resistance Readings from an ENDOHM
    November 18, 2013
    One of our frequently asked questions (FAQs) concerns TEER measurements with an EndOhm. If the resistance readings from your ENDOHM don't stabilize, you may need to do some troubleshooting. Test the EVOM2 First, test your EVOM2 meter. The 1000Ω test resistor (WPI # 91750) can be used for this purpose. Insert the RJ-11 plug at the end of the test resistor  into t
  5. STX100 Electrodes TEER Measurement
    October 11, 2013
    With the development of a High Throughput Screening (HTS) protocol for faster drug discovery, a new line of cell culture filter plates have been introduced by several major cell culture insert manufacturers. These HTS plates normally have either 24 or 96 individual cell culture inserts "bonded" together as one plate so that it can be handled by a robot apparatus. In response to these developments, WPI has developed an automatic REMS system and a manual electrode, STX100, for TEER measurements using HTS plates. Designed for use with 24-well HTS plates (Corning Costar and BD Falcon) and with 96-well plates (Millipore and BD Falcon) Improved accuracy down to
  6. TEER Research: WPI's Manual and Robotic Systems
    October 11, 2013
    Transepithelial Electrical Resistance TEER measurements is the most convenient, reliable and non-destructive method for evaluating and monitoring the growth of epithelial tissue cultures in vitro. The confluence of the cellular monolayer is quickly determined by a sharp increase in TEER. TEER measurement technology, which was first introduced by WPI in the mid-1980's, has since been perfected and expanded to include a range of TEER related manual and automatic instrumentation, including: EVOM² - Manual TEER measurement of epithelial cells in 24- and 96-well plates REMS AutoSampler - Automated system for High Throughput Screening (HTS) EVOM2 Volt-Ohm Meter
  7. REMS Auto Sampler - HTS Robot
    October 11, 2013
    The REMS AutoSampler automates measurements of electrical resistance of transepithelial, transendothelial or Caco-2 cell membranes being grown to confluence on microporous filters of high throughput screening (HTS) 24- and 96-well microplates. It is a computer-controlled, tissue resistance measurement system that offers reproducibility, accuracy, flexibility and ease-of-operation for this kind of measurement. Automated measurement of tissue resistance in cell culture microplates provides the important advantages of speed, precision, decreased opportunity for contamination and the instant availability of measured resistance data on a computer. These measurements are useful in applications such as drug bioavailability studies and studies on the mechanisms of
  8. How to Use an EndOhm Chamber with an EVOM2
    September 03, 2013
    The EVOM2 measures trans epithelial electrical resistance (TEER) in tissue culture research. The EndOhm series of chambers for the EVOM2 are designed to accurately measure trans endothelial electrical resistance. Learn how to setup your EndOhm Chamber with an EVOM2 Epithelial Volt Ohm Meter. See how to equilibrate the sensors when taking a voltage measurement. And, learn how to take resistance and voltage measurements.  
  9. EVOM2 Remix
    April 30, 2013
    EVOM was the first instrument designed to perform routine Trans Epithelial Electrical Resistance (TEER) measurement in tissue culture research. EVOM2 is the next generation, redesigned for greater useability. The EVOM2 sports the following features: Rechargeable Battery - EVOM2 , which plugs into a standard wall outlet, comes with a NiMH 6V 200mAH rechargeable battery backup. The battery charges whenever the unit is plugged in. If the battery runs low, the EVOM2 automatically shuts down. Simply install the supplied power cord and plug it into the wall outlet. The battery will fully charge in 24 hours. Normal laboratory testing can continue while the EVOM2 recharges.
  10. How To Test Your EVOM2 Meter
    April 24, 2013
    The World Precision Instruments (WPI) EVOM2is used for making trans epithelial electrical resistance (or TEER) measurements. We recommend that you test your EVOM2 before you use it for the first time and then periodically after that. If there is a concern that the meter or the electrode is not functioning properly, test the meter first. This video shows how to test the meter. More Info
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