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Epithelial Physiology
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May 02, 2023
The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM™ technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.
The video below shows details the features and functions of the Experiment window on the EVOM™ Auto:
After you have defined a sequence, you can begin running an experiment.
The Experiment window shows a representation of the plate on the left side of the window.
On the right side of the window, you see these...more
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April 27, 2023
The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM™ technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.
The video below shows you how to schedule an operation on the EVOM™ Auto:
The data acquisition functions of the EVOM™ Auto may be repeated at a defined time interval when multiple scans of a plate are needed. The scheduled operations are displayed on the right side of the Experiment window in the Current State area. The control buttons for scheduling...more
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April 25, 2023
The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.
The video below shows you how to stabilize the electrode array on the EVOM™ Auto:
First, select Maintenance on the main menu. To stabilize, press the + or - buttons to set the timer to the desired time in minutes in the stablize frame.
Next, Select the radio button for the rinse station containing the buffer solution. Then, fill the appropriate rinse station with the buffer solution...more
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April 20, 2023
The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM™ technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.
The video below shows you how to chloride the electrode array on the EVOM™ Auto:
To chloride the electrode array, select Maintenance on the main menu.
Four control buttons are located at the bottom of this window:
- Go Home- Positions the electrode array in the home position.
- Calibrate- Calibrates the sensor head using the internal...more
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April 18, 2023
The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM™ technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.
The video below shows you how to create a plate file on the EVOM™ Auto:
1. To Create a measurement sequence, select the Manage Sequence menu from the Home screen. A list of the existing sequences displays, and you may open and edit any existing sequence or select New Sequence to create a new one. If you select New Sequence, a popup allows you to...more
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April 13, 2023
The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM™ technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.
The video below shows you how to download and how to delete a data file EVOM™ Auto:
Once a data acquisition run is complete, the data may be exported. This process exports all the runs performed on a plate into a downloadable, comma-delimited (CSV) file which can be read by Microsoft® Excel or Google Sheets. The downloaded file is immediately available.
To download a data file, press the Download...more
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April 11, 2023
The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM™ technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.
The video below shows you how to run an experiment on the EVOM™ Auto:
To begin running an experiment, select Experiment from the main menu. Then, select the Plate Name drop-down in the Details area on the right side of the screen. Choose from the list of existing plates or select New Plate to define a new plate. Enter the name for the plate...more
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April 06, 2023
The EVOM™ Auto automates measurements of TEER in epithelial or endothelial monolayers cultured on high throughput screening 96-well plates utilizing our innovative EVOM™ technology, qualitatively measuring cell monolayer health and quantitatively measuring cell confluence by determining an increase or a plateau in tissue resistance.
The video below shows you the features of the homepage on the EVOM™ Auto:
Introducing the Home Screen – Tips for Getting Started
Select a Plate Brand
The Electrode Array is configured to support 96-well plates. Three different brands of plates are supported:- C96_HTC – Corning 96 Well Plate
- M96_HTC – Milipore 96 Well Plate
- O96_HTC...more
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April 04, 2023
Optimized, in vitro workflow to identify compounds with vasoactive properties
In vitro models have employed two common methods to quantify changes in endothelial barrier integrity: transepithelial/transendothelial electrical resistance (TEER) and tracer compound permeability.1 TEER is a non-invasive method that quantifies changes in electrical conductance to measure confluency and barrier integrity. Tracer compound permeability uses molecules of defined molecular weights to measure the size exclusion capacity of cell barriers (e.g., 4 kDa FITC-dextran or FD4).1 Using the EVOM™ Manual (EVOM-MT-03-01) with the EndOhm TEER electrode and cell culture permeable supports, this application note describes how to non-invasively evaluate endothelial barrier integrity after cytokine treatment and provides a method to identify vasoactive compounds that have the potential to induce vascular injury. Tracer compound permeability studies...more
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March 16, 2023
World Precision Instruments' (WPI) legacy Robotic Epithelial Measurement System (REMS) and the new advanced, upgraded version of REMS, EVOM™ Auto, are high-throughput, automated transepithelial/transendothelial electrical resistance (TEER) measurement systems. These robotic systems, combined with EVOM™ technology, expedite TEER measurements.
A 96-well plate can be read within a few minutes using an automated TEER measurement system versus using a manual TEER measurement system, which can take up to 90 minutes to read a single 96-well plate. Manual systems require a user to move the electrode from one well to another and perform the rinsing in between, if needed. (Rinsing of the electrode while reading through a number of samples helps to prevent sample cross-contamination.) As automated systems measurements and rinsing are controlled by robotics, manual handling time is eliminated. Additionally, automated systems offer better measurement...more
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March 03, 2023
Have you got the new EVOM Auto TEER Measurement System for 96-well HTS plates? This short videos shows how easy it is to setup.
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September 13, 2022WPI is very excited to announce our newest product launch and collaboration with MatTek Life Sciences. The companies will offer both MatTek PermaCell Cell Culture Insert Plates and compatible customized World Precision Instruments’ STX HTS EVOM™ Electrode for TEER to their respective customers, available immediately to the life sciences market...more
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July 19, 2022
EVOM™ Manual is WPI's newest instrument to measure Trans Epithelial Electrical Resistance (TEER). Let's look at what comes with the system and how easy it is to setup.
The EVOM™ Manual comes with the following components in the box:
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July 19, 2022
EVOM™ Manual Monitors Cellular Health
WPI's EVOM system is popular in the research community, both in academia and in industry, and it is commonly used for the evaluation of mammalian cellular health by measuring transepithelial/transendothelial electrical resistance (TEER or TER) of cellular layers.
EVOM™ Manual is powered by the same EVOM™ technology as older EVOM models (EVOMX, EVOM, EVOM2 and EVOM3). It has advanced features for performing experiments more easily. With the new touchscreen display you can now store data as Microsoft® Excel files on a USB flash drive. Just remove the flash drive with all your recorded data from the EVOM™ Manual and plug it into a computer to access and plot your data...more
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July 13, 2022
EVOM™ Manual is WPI’s newest instrument to measure Trans Epithelial Electrical Resistance (TEER). Let’s look at how to set it up and take voltage measurements.
- Power on the EVOM™ Manual using the power switch on the rear panel.
- Insert the USB flash drive provided with the system into the USB port on the side of the meter.
- By default, the filename is plate1. You may choose a prefix that auto-indexes or a unique file name:
- Press and hold...more
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