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Data Acquisition

  1. Data Acquisition Designed for Muscle Physiology Pre-Clinical Trials
    October 20, 2015
    LabTrax 8/16 hardware combined with MDAC software is an excellent choice for WPI's SI-H line of muscle physiology products like the SI-MT Muscle Platform, SI-CTS Cell Tester and SI-HTB Horizontal Tissue Baths. This data acquisition system is ideal for testing physiological characteristics like the contractile and elastic properties of different tissues in various conditions like isometric, anisometric or isotonic states. This data acquisition system was designed with the muscle researcher in mind, because the knowledge of physiological characteristics of muscles is critical when quantifying the beneficial or adverse effects of a stimulus (like a pharmaceutical drug) on muscle function in pre-clinical studies.  Up to 8 Channels with Independent Control LabTrax 8/16 is a powerful low noise and high resolution data acquisition system, allowing up to 8 ADC channels and 4 DAC channels synchronously for user-defined muscle length and electrical stimulation protocols. It includes a number of adjustable standard functions like DC levels, ramps, square waves, sinus waves and staircases so you can define your own muscle length and electrical stimulation protocols. These standard functions can be combined in any configuration to create specific, user-defined, experimental protocols. In addition, two 5V TTL standard BNC outputs are available to drive further devices. Thanks to its global feature in defining numerous lengths change and electrical stimulation protocols, and its standard BNC connections, LabTrax 8/16 with MDAC can drive external devices.  Experimental Protocols in Muscle Physiology In muscle physiology, specific experimental protocols can be programmed to measure muscle contractile and elastic properties like twitch or tetanus protocols (for example, cardiac or skeletal muscle), quick stretch-relaxation protocols to calculate dynamic force or static force, or sinusoidal perturbation protocols (for example, lung tissue mechanics) to describe force-length loops to obtain dynamic stiffness and hysteresis effects of biological tissue. In addition, fatigue protocols may be individually programmed.   Continuous Stimulation for Cardiac Cells New features of LabTrax 8/16 with MDAC software include the use of continuous stimulation protocols to maintain especially cardiac cells during resting periods. Annotating the Data Annotation marks can be set during acquisition and read in the analysis options. Data Analysis Included with Data Acquisition Software MDAC software also includes a standard offline Analysis Toolbox for data processing and data analysis. Data processing and data analysis methods can be applied to each single channel individually. Software Filters for Data MDAC data processing allows for the use of different filter options to smooth the signal or to delete frequency components, using adjustable Butterworth low-pass, high-pass, band-pass or band-stop filters. MDAC includes the use of adjustable smoothing filters like Savitzky-Golay or Moving average. Down sampling options further smooth the signal and reduce the data amount. Mathematical and Statistical Calculations of Data MDAC data analysis allows for running mathematical operations on the acquired signals, like the calculation of the derivative or integral. In addition, the derivative can be calculated on the maximum or minimum value of the signal or on a value of a specific cursor position. Within these options, the calculation of a slope value for a given signal or the latency between two signals is possible. Finally, standard statistical analysis within MDAC can calculate the mean or median value for a selected interval and its corresponding standard deviation, as well as, the minimum and maximum value within the interval. For all data processing and data analysis methods, the raw data set can easily be restored during analysis, while already processed data can be stored as binary file. Further automated standard analysis, like twitch or tetanus, or customer defined analysis routines may be included as specific toolboxes. Excellent Data Acquisition System for Muscle Physiology Applications MDAC software combined with LabTrax 8/16 hardware is a reliable and affordable solution for muscle physiology applications.    More Info
  2. Recording TEER Measurements from an EVOM2
    November 11, 2014
    The EVOM2 is the classic, hand-held instrument for making Trans Epithelial Electrical Resistance (TEER) measurements. The REMS system adds a robot and data recording for automated TEER measurements. There may be times when you don't have a robot, but would still like to have the data recording capabilities of the REMS system. With a little ingenuity, you can do just that. Here, we will show you how you can add data recording to your standard EVOM2. Open Labscribe3 on your computer. Let the program identify the data recorder. In our example, we will use a Lab-Trax-4. Choose the default settings. Setup the preferences for one channel. If you like, you may add five points of smoothing.  In the marks section of the main window, select A1 (channel one). This records a data point directly into the marks records window. Alternatively, the smoothed graph C1 may be selected. In the top graph, right click and choose Units>Simple to calibrate the graph to ohms. Enter these values for the EVOM2 conversion: 2 point calibration Apply units to new data Value at Cursor 1: 0.0000 => 0.0000 Value at Cursor 2: 1.0000 => 1000 Unit Name = Ohms To connect the EVOM2 to the data recorder, connect a BNC cable from the Output BNC on the front of the EVOM2 to the Channel 1 input BNC on your data recorder. In the Marks window, enter A1 or Control. Move the electrode to the control well, and allow it to stabilize. Then, press Enter. Otherwise, it is difficult to hold the electrodes still while typing. For each additional well, enter in the number A2…, A3 into the Marks field. Then, move the electrode to that well and press the enter key after the reading stabilizes. Stop the recording and save the file. Open up the marks editor in Labscribe (View>Marks>Marks). The dialog box shows the time mark data and resistance values.   Press the Export button and choose “All Marks.” Give your export file a name. The export files is a text file that may be import into Microsoft Excel or another spreadsheet application.
  3. LabScribe Software for LabTrax
    May 01, 2013
    LabScribe is the powerful software included with Lab-Trax™ (formerly called DataTrax). The acquisition and data display in LabScribe is easy and straight forward. It supports real units and a time-based display that is not coupled to sample rate. You can display up to 1,000,000 data points per channel on the screen. Scrolling, zoom-in and zoom-out tools, together with a searchable list of user interventions, make finding important areas of data easy. And, of course, you can print or export anything that you see. The Main Display Data recorded with LabScribe is presented as distance-per-unit of time (for example, cm/sec) exactly as it would be on a traditional paper chart recorder. The x-axis display is compressed or expanded using point and click icons in the LabScribe toolbar. LabScribe ’s display can smoothly scroll data at any speed. The display scroll rate is not determined by sampling rate, so you have full control over resolution and display. This is particularly useful when you want to compress high-resolution data on the time axis to create a data overview. Currently the software can display 100,000 points per channel online and one million points per channel offline, so you can see quite a bit of data at a glance. Main display window showing all eight channels. LabScribe ’s Main Window currently supports functions that can show you the real-time rate, integral or any of 16 online analyses. Each of these is set up from a single click in the Main Window. LabScribe also has an “Analysis Window” where students can make more in-depth measurements and build lab reports. There are 31 different kinds of measurements supported in this window. AutoScale A single button allows you to find the data on a channel, wherever it is, center it and expand it to fill the available channel space. AutoScale used with high-resolution recorders ensures that you will never again lose data because they are “out of range or off scale.“ Cursors Most measurements students make on recorded data involve a distance in time or a difference in amplitude. For this reason, LabScribe places two easy-to-move cursors in each display of data. LabScribe displays the difference in time and the difference in voltage (or calibrated units) between the cursors. Cursors can be moved with the mouse, or for precise placement, with the arrow keys on the keyboard. Real World Units LabScribe allows you to calibrate the displayed data in any unit that you choose. Reading your data in mmHg or grams or micro amps takes the guesswork out of analysis. Real-Time Marks Researchers can insert “Text Marks” from the keyboard into their data to indicate when events like drug delivery or stimulus points are occuring. Marks are easy to search and the ”Go To Mark” feature will take you to a selected event from anywhere in the data. For reporting, researchers can position the text in a given mark over the data it applies to, just like writing on chart paper. Marking window. Twin DACs (WPI-118 only) LabScribe allows you to program two independent analog outputs. They can be used as independent stimulator outputs, or as linear controls for pumps or other hardware that accept an analog voltage as control. Built-in wave protocols include pulse, step, pulse train and DC. The two outputs can be combined to produce more complex protocols. Stimulator window. Digital Inputs Up to eight digital inputs can be configured from LabScribe. Raw data, count frequency and open times can be displayed in real time. Digital Output Up to eight digital outputs can be configured from the LabScribe software. Digital outputs can be used to control valves or relays in the experimental rig, or they can be configured in parallel to control serial devices. Output sequences of events are created from an easy-to-use sequence builder that can include both the digital outputs and the analog outputs. Sequences are remembered by LabScribe and can be called manually or automatically. Input/Output window. Lab Settings Settings or templates for the various lab protocols are stored in the program’s settings menu. Each setting can include a Lab procedure and Journal format, as well as the program acquisition and analysis settings. This makes changing program settings to accommodate different experiments point-and-click easy. Free Upgrades and Built-in Site License LabScribe software upgrades are free. Better yet, LabScribe may be freely distributed and copied to other lab computers. That means that researchers can record data in the lab and work on analysis and lab reports at their leisure in different locations. LabScribe is provided on an open license so you can record data in the lab and analyze it in your office without buying multiple copies of the software.   More Info
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