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Biofluorometer

  1. Light Engine - Turning Light into Science
    April 20, 2015
    If you have seen a rainbow, you have seen the visible part of the light spectrum spread out. Each color we see is actually a different wavelength of light. In scientific applications, we are often interested in only one or two wavelengths of light. For example, a muscle tissue can be stained with Indo-1 or Fluo-4 causing intra-cellular calcium to fluoresce when excited by the appropriate wavelength of light. Or, a neuronal cell can be stained with di-4-ANEPPS and di-8-ANEPPS, causing a fluorescent signal that corresponds with the firing of an action potential. WPI’s new SI-BF-100 Biofluorometer is a light engine that generates quick pulses of light in one (or
  2. SI-BF-100 - The Perfect Fluorometer for a Host of Measurement Applications
    July 07, 2014
    Gabe and Alec discuss the breakthroughs in fluorescence measurements that cut the cost and the complexity of traditional systems using LED technology instead of mechanical filter wheels, filters and costly lamps. More Info
  3. Measuring the Relationship between Force, Energy Consumption and Calcium Concentrations in Muscle Fibers using WPI-SIH Research Systems
    February 12, 2014
    Muscle contraction and relaxation is caused by the attachment and detachment of two types of molecules (actin and myosin) to each other within the fibers that compose a muscle. These molecules are arranged as overlapping filaments within the functional units of the muscle fibers that are known as sarcomeres. Each crossbridge that is made between the end of a myosin molecule and a binding site on an actin molecule requires a molecule of ATP, an energy source, to be hydrolyzed when the end of the myosin molecule is released from the actin filament. After the release, the myosin molecule is ready to move down to another actin binding site causing the sarcomere, and the muscle, to shorten. When ATP is regenerated through a series of enzymatic reactions, another molecule, NADH, provides the energy needed for the regeneration of the ATP. The NADH fluoresces when exposed to ultraviolet (UV) light. The oxidized form of the molecule NAD+, which has lost its stored energy, does not fluoresce.
  4. High Intensity LEDs Allow New Applications for the Biofluorometer
    November 07, 2013
    The new SI-BF-100 is an LED-based fluorometer for life science applications. With up to seven LED modules (wavelengths), the SI-BF-100 covers many fluorometric applications in both Neuroscience and Cell Biology. This technology significantly cuts the cost of fluorescent imaging without sacrificing resolution or quality. WPI's recent introduction of the high intensity LED version of the Biofluorometer opens a host of new applications for life science researchers.  In Vivo Applications Monitor electrical activity over large areas of intact brain without the limitations of microelectrode arrays Simultaneously monitor electrical activity an
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