Laboratory Equipment Blog
The use of fluorescence for sensing and imaging of the cellular signaling pathways has emerged as an indispensable tool in modern physiology, providing dynamic information of quantity and localization of the molecules of interest. Using appropriate indicator dyes, molecules alter their fluorescent characteristics in response to ion binding or membrane integration, so that the optical signal from the indicator can be measured to monitor the amplitude and the time course of various metal ions like Na+, K+, Mg2+ and Ca2+, as well as pH and membrane potential, in cellular compartments.
Absorption of light correlates to the energy of a photon that is taken-up by electrons of the substance atom. The electromagnetic energy is transformed into internal energy of the absorbent substance. The absorbance of a substance quantifies how much of the incident light is absorbed by it (instead of being reflected or refracted). Precise measurements of the absorbance at many wavelengths allow the identification of a substance via absorption spectroscopy, where a sample is illuminated from one side, and the intensity of the light that exits from the sample in every direction is measured (see Fig. 1). A few examples of absorption are ultraviolet–visible (UV-Vis) spectroscopy or infrared (IR) spectroscopy.
The use of fluorescent probes in cell physiology has emerged as indispensable tool in the analysis of cell functioning over recent years. The physics underlying fluorescence is illustrated by the electronic-state diagram (so-called Jablonski diagram, see Fig. 1), showing the three-stage process to create the fluorescent signal (Excitation - Excited/State Lifetime - Fluorescence Emission) in a fluorophore/indicator and simplified described below.
Cuvettes come in a variety of shapes and sizes, but one of the most important specifications of a cuvette is its Z-dimension. The Z-dimension of an instrument (cuvette holder or spectrometer) is the distance from the bottom of the cuvette chamber floor to the center of its light beam (see image). A cuvette’s Z-dimension must match the Z-dimension of the instrument with which it will be used.
Surgical Loupes help to alleviate eye strain by enlarging the image when you are working on tiny subjects or conducting precision operations. They are portable and easier to use than a surgical microscope. However, they are not created equal, and choosing the pair that's right for you is important to your satisfaction.
Inox, Titanium, Dumoxel®, Dumastar®, Antimagnetic... Have you ever looked at the variety of metal alloys for surgical instruments and laboratory tools and wondered which is best for your needs? Here's a brief rundown.
Cures Acne! Relieves arthritis, dysphasia and chronic pain! Speedshealing of inflamed muscles! Melts belly fat! Even used for treating cancer!The claims are so broad and general that it sounds too good to be true. Yet, thescience behind the new, non-invasive treatments is fascinating. While scientistcontinue to explore how electromagnetic fields and electric stimulation affecttissue, the science behind infrared therapy is solid.
Find out what you need to know before you buy your next amplifier. WPI amplifiers consistently outperform the cheaper competitors, because WPI reduces the schott noise, which provides a cleaner output. Read this article to learn how an amplifier works so you will know what to look for when you are buying your next amplifier.