Application Notes
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June 30, 2023
In any laboratory, having key lab supplies is almost as important as having the major equipment. Choosing a reputable supplier of these necessary supplies is as important as having quality laboratory supplies when you need them. WPI wants to be your partner in early drug discovery, and we stock a wide variety of lab supplies, many of which can ship the same business day. Having a variety of lab supplies ready to ship makes us a dependable research partner. Here are some of the popular supplies that we keep on hand to meet your needs for your upcoming experiment...more
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May 01, 2023
Improved User Experience
EVOM™ Auto is the latest generation of WPI’s automated transepithelial or transendothelial electrical resistance (TEER) measurement system. Using the same proven technology in the EVOM™ Manual and REMS, combined with a new multi-electrode array, software interface and control system, it delivers our fastest workflow solution while improving TEER measurement accuracy: - Faster throughput – read a 96-well plate in under 3.5 minutes (A 2-rinse cycle can be completed in 7 minutes, almost halving the time compared to a REMS)
- Automatic sample averaging improves accuracy and stability
- Compact size for easy set up and operation inside in a hood or incubator
- Wireless control of the autosampler
- Smart user interface and web...more
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April 04, 2023
Optimized, in vitro workflow to identify compounds with vasoactive properties
In vitro models have employed two common methods to quantify changes in endothelial barrier integrity: transepithelial/transendothelial electrical resistance (TEER) and tracer compound permeability.1 TEER is a non-invasive method that quantifies changes in electrical conductance to measure confluency and barrier integrity. Tracer compound permeability uses molecules of defined molecular weights to measure the size exclusion capacity of cell barriers (e.g., 4 kDa FITC-dextran or FD4).1 Using the EVOM™ Manual (EVOM-MT-03-01) with the EndOhm TEER electrode and cell culture permeable supports, this application note describes how to non-invasively evaluate endothelial barrier integrity after cytokine treatment and provides a method to identify vasoactive compounds that have the potential to induce vascular injury. Tracer compound permeability studies...more
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March 03, 2023
Introduction
In the process of ‘cell cycle’, cells grow and divide into two genetically identical daughter cells. It is regulated by a complex signaling pathway which keeps cell homeostasis by regulating cell division and DNA duplication1. On the other hand, because cancer cells grow and divide indefinitely out of cell cycle control, anti-mitotic drugs are used to suppress abnormal proliferation...more -
March 01, 2023
Introduction
Cytotoxicity refers to the degree of damage to cells.
caused by chemical substances or physical factors. Measuring it through cytotoxicity assay is essential for drug development and biological research. Cells undergo complex signaling pathways that causes various cell death processes such as apoptosis, necrosis, and necroptosis. However, most cytotoxicity assays...more -
February 24, 2023
EVOM™ AutoLCI Automated Live Cell Imaging System
An integrated live cell monitoring system for stable imaging of suspension cells in immunological research
As white blood cells responsible for immune function are suspension cells that travel along blood vessels, immunology studies often use various suspension cell lines originating from white blood cells. Dealing with suspension cells, unlike adherent cells, slight movement of a plate when locating it on the microscope causes the cells to float. Aside from the problems caused by temperature and CO2 instability, it is in fact not possible to use a traditional microscope to monitor cells in real time. Therefore, in order to stably monitor suspension cells, a live cell imaging device such as EVOM™ AutoLCI that operates...more -
August 21, 2019
Are you looking for a microliter or sub-microliter and high precision syringe that holds needles as small as 36 gauge (G), in addition to having the capability to connect to quartz tubing?
WPI’s NanoFil is the answer. We offer NanoFil syringes with NanoFil needles or the option to connect the NanoFil syringe to quartz tubing to use in research studies, mainly involving sub-microliter volume injections into animal tissues...more
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December 08, 2014
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October 21, 2014
A low-noise amplifier like the DAM50 is an excellent choice for EEG recording in rodents. WPI's amplifiers were engineered for the bio-medical researcher. While 20-30μV of noise is common in bio-amplifiers, WPI’s DAM series amplifiers generate 0.4μV RMS (root mean squared) at 0.1-100Hz. (That’s equal to about 2μV peak to peak.) This setup shows one way such recordings could be made. The RC1 electrode works well for rats, and the EP1 is more suitable for mouse cranial application.
For this application, you will need the following equipment:
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July 08, 2014
Watch how researchers from the University of Chicago inject adult zebrafish using a 10μl NanoFil microsyringe controlled by a Micro4 controller and UltraMicroPump III (UMP3-1 includes one UMP3 pump and a Micro4 controller).
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July 01, 2014
In this JoVE video you can observe as the researchers perform an intraluminal middle cerebral artery occlusion (MCAO) on a mouse. The MCAO model is the most frequent used model among experimental ischemic stroke models.
WPI instruments used in this application include:
SuperCut Iris scissor, straight 14218 Vannas micro-scissor, straight 14003 Dumont fine tip forceps 503290 Vessel -
June 25, 2014
Chiara Cianciolo Cosentino, at the University of Pittsburgh, describes how she uses intravenous microinjections of zebrafish larvae to study acute kidney injury in this JoVE video. You can also watch this video on JoVE. WPI equipment shown in this video includes:
- MMP Manual Microsyringe Pump
- PV820 Pneumatic PicoPump
- M3301 Manual Micromanipulator
- M10 Magnetic Stand
- 5052 Steel Base Plate
...more
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October 03, 2013
WPI surgical instruments were recently featured in a JoVE video that demonstrates a new method for cross pollinating grasses.
Jiang, H., Barbier, H., Brutnell, T. Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses. J. Vis. Exp. (80), e50527, doi:10.3791/50527 (2013)...more
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August 23, 2013
Researchers at the University of Michigan are using WPI's PV820 for injecting a morpholino solution into the lumen of the otic vesicle of 1-day old zebrafish embryos. Then, they use electroporation to introduce mif and mif-like morpholinos into the developing inner ear tissues.
Check out the ...more
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May 22, 2013