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Laboratory Equipment Blog

  1. How the SI-BAM21-LCB Amplifier Works
    February 13, 2014
    In a typical muscle physiology setup, a muscle is held by a force transducer. The force transducer is connected to the SI-BAM21-LCB. As the muscle contracts or releases, the transducer converts the force into an electrical current signal which is proportional to the force applied to the transducer. The SI-BAM21-LCB converts the current signal into a voltage signal that can be displayed on the screen of the recording device. Before initiating an experiment, the SI-BAM21-LCB must first be zeroed. This sets the baseline for measurements to follow. The output signal is buffered and multiplied by 1, 2, 5 or 10, depending on the Gain switch setting on the front panel of the amplifier
  2. Eliminating the Resonance Frequency with the Anti-Oscillation Unit
    February 13, 2014
    Every force transducer has a resonance frequency at which it vibrates. The SI-AOSUB allows you to locate that frequency and filter the signal to mitigate the noise of the resonance frequency. Since each force transducer is unique, the anti-oscillation unit must be calibrated for each force transducer. Likewise, the tissue mounting hardware affects the resonance frequency. Therefore, the system must be calibrated with the mounting hardware attached to the force transducer. The SI-AOSUB Anti Oscillation module operates in conjunction with the SI-BAM21-LCB module and the motor controller to actively cancel the force transducer’s natural harmonic resonance. This is critical when performing dynamic mu
  3. Constant Load Modes Available for Muscle Physiology Experimentation
    February 13, 2014
     The Constant Load Module offers three modes of operation, including: Constant Load External Loop Bypass This block diagram (right) graphically shows the three modes of operation in the Constant Load module. Constant Load–This mode maintains a constant load on the tissue sample. SI-AOSUB Corrected Output, representative of the force transducer output, is the SI-COLUB feedback input. The SI-AOSUB Corrected Output is connected to the CL CMD (constant load command). An in
  4. Measuring the Relationship between Force, Energy Consumption and Calcium Concentrations in Muscle Fibers using WPI-SIH Research Systems
    February 12, 2014
    Muscle contraction and relaxation is caused by the attachment and detachment of two types of molecules (actin and myosin) to each other within the fibers that compose a muscle. These molecules are arranged as overlapping filaments within the functional units of the muscle fibers that are known as sarcomeres. Each crossbridge that is made between the end of a myosin molecule and a binding site on an actin molecule requires a molecule of ATP, an energy source, to be hydrolyzed when the end of the myosin molecule is released from the actin filament. After the release, the myosin molecule is ready to move down to another actin binding site causing the sarcomere, and the muscle, to shorten. When ATP is regenerated through a series of enzymatic reactions, another molecule, NADH, provides the energy needed for the regeneration of the ATP. The NADH fluoresces when exposed to ultraviolet (UV) light. The oxidized form of the molecule NAD+, which has lost its stored energy, does not fluoresce.
  5. SI-CTS200 has a Rotating Cuvette
    February 12, 2014
    The SI-CTS200 system utilizes a unique rotating bath to dramatically improve experimental throughput. The rotating bath is designed to orient cells in the XY plane so that no physical manipulation of the position of the cell itself is required prior to capture by the grabbing devices attached to the force sensor and linear actuator.   The cuvette rotates to allow for precise positioning of the cells to be mounted. This bath has two interchangeable inserts. The first holds any 35mm glass bottom dish (WPI #FD35-100). When coating tweezers or glass rods with a biocomaptible adhesive, insert a FluoroDish into the holder and place it in the rotating cuvett
  6. WPI Featured in Local Publication
    November 20, 2013
    WPI was recently featured in the Sarasota Daily. You can read the article here.
  7. FAQ: Troubleshooting Unstable Resistance Readings from an ENDOHM
    November 18, 2013
    One of our frequently asked questions (FAQs) concerns TEER measurements with an EndOhm. If the resistance readings from your ENDOHM don't stabilize, you may need to do some troubleshooting. Test the EVOM2 First, test your EVOM2 meter. The 1000Ω test resistor (WPI # 91750) can be used for this purpose. Insert the RJ-11 plug at the end of the test resistor  into t
  8. High Intensity LEDs Allow New Applications for the Biofluorometer
    November 07, 2013
    The new SI-BF-100 is an LED-based fluorometer for life science applications. With up to seven LED modules (wavelengths), the SI-BF-100 covers many fluorometric applications in both Neuroscience and Cell Biology. This technology significantly cuts the cost of fluorescent imaging without sacrificing resolution or quality. WPI's recent introduction of the high intensity LED version of the Biofluorometer opens a host of new applications for life science researchers.  In Vivo Applications Monitor electrical activity over large areas of intact brain without the limitations of microelectrode arrays Simultaneously monitor electrical activity an
  9. STX100 Electrodes TEER Measurement
    October 11, 2013
    With the development of a High Throughput Screening (HTS) protocol for faster drug discovery, a new line of cell culture filter plates have been introduced by several major cell culture insert manufacturers. These HTS plates normally have either 24 or 96 individual cell culture inserts "bonded" together as one plate so that it can be handled by a robot apparatus. In response to these developments, WPI has developed an automatic REMS system and a manual electrode, STX100, for TEER measurements using HTS plates. Designed for use with 24-well HTS plates (Corning Costar and BD Falcon) and with 96-well plates (Millipore and BD Falcon) Improved accuracy down to
  10. TEER Research: WPI's Manual and Robotic Systems
    October 11, 2013
    Transepithelial Electrical Resistance TEER measurements is the most convenient, reliable and non-destructive method for evaluating and monitoring the growth of epithelial tissue cultures in vitro. The confluence of the cellular monolayer is quickly determined by a sharp increase in TEER. TEER measurement technology, which was first introduced by WPI in the mid-1980's, has since been perfected and expanded to include a range of TEER related manual and automatic instrumentation, including: EVOM² - Manual TEER measurement of epithelial cells in 24- and 96-well plates REMS AutoSampler - Automated system for High Throughput Screening (HTS) EVOM2 Volt-Ohm Meter
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