Laboratory Equipment Blog

DNA/RNA Quantification Using a 2mm Cuvette and a Tidas Spectrometer

Posted: Wednesday, May 1, 2013

Concentrations of DNA in solution (31µg/mL and 561µg/mL) were measured with a spectrometer and UV/Vis light source in a cuvette. A 2mm pathlength cuvette does not require a pre-measurement dilution within this concentration range, thus a potential source of error was eliminated. Although a 2mm cuvette has a total internal volume of 0.7mL, only 350µL is required to obtain an accurate measurement.


Z-Dimensions Are Not Created Equal

Posted: Wednesday, May 1, 2013

Z-DimensionCuvettes come in a variety of shapes and sizes, but one of the most important specifications of a cuvette is its Z-dimension. The Z-dimension of an instrument (cuvette holder or spectrometer) is the distance from the bottom of the cuvette chamber floor to the center of its light beam (see image). A cuvette’s Z-dimension must match the Z-dimension of the instrument with which it will be used.

 


Choosing a Cuvette: Optical Glass or Quartz

Posted: Tuesday, July 12, 2011

Synthetic quartz can be used in deep UV applications and is recommended for fluorescent applications, as it does not exhibit background fluorescence. Quartz cuvettes (absorbance, fluorescence and flow) are shipped individually packaged, glass cuvettes are shipped in packages of 10 cuvettes.