Laboratory Equipment Blog
If you have seen a rainbow, you have seen the visible part of the light spectrum broken out. Each color of light is actually a different wavelength of light. In scientific applications, we are often interested in only one or two wavelengths of light. For example, a muscle tissue can be stained with a dye that makes the calcium glow when a certain wavelength of light hits it. Or, a nerve cell can be stained with a dye that glows when the cell is firing.
Measuring the Relationship between Force, Energy Consumption and Calcium Concentrations in Muscle Fibers using WPI-SIH Research Systems
Historically, a light source, filter wheel and photomultiplier were used to excite the dye at the proper wavelength. Now, using LED modules, we have much greater control over the measurement cycle. You can set the length of time each LED fires and establish the firing sequence. In addition, you can define the intensity of the LED signals, determine the number of readings used to calculate the rolling average of the ratio displayed, and set the photomultiplier filter frequency.
WPI's new Biofluorometer is now available with high intensity LED modules, opening a wide variety of new applications in cellular biology and neuroscience.