Setting up OxyMicro and OxyMini

A New Generation of Fiber Optic Oxygen Sensors Based on Luminescence Lifetime

Oxygen measurement is simpler than ever. Just stick a disposable, oxygen-sensitive "spot" to the inside of a flask, beaker, test tube or bottle, and fill the container 

OXY-MINIwith the solution to be tested. Then, on the outside of the glass container, hold the fiber optic wand close to the spot to take a reading. As the spot reacts with oxygen, it gives off light, which is measured with the fiber optic wand. This ingenious system is highly accurate and affordable. Two different units (which use the same operating principles are available: OXY-MINI and OXY-MICRO. 

The OXY-MINI system is optimized for process control and biotechnology applications. The OXY-MICRO is designed for biological research applications including OXY-MICROimplanting into tissues, cell cultures, profiling of biofilms and sediment related bioassays. The measurement principle of the sensor system is based on the detection of oxygen concentration as a function of luminescence lifetime either in dissolved or gaseous phase environments.

Features

  • Oxygen is not consumed during the experiment
  • Immune to electrical and magnetic interference
  • Excellent long-term stability
  • No lengthy polarization necessary (for example, as Clark-type oxygen electrodes require)
  • Fast response time < 0.5 s for MicroTip sensors
  • Probe size of MicroTip sensors as small as 50µm
  • Measurement is feasible in dry gas
  • Optical isolation of sensor tip available for fluorescent or photosynthetically active samples

Meters

The OXY-MINI  and OXY-MICRO  fiber optic oxygen meters are compact, easy to transport. The instruments are designed for in/outdoor use and can be connected to a PC via a RS232 interface. Data can be visualized, analyzed and stored with the supplied software. A full range of sensors covering most biomedical applications are available.

OXY-MICRO System

The OXY-MICRO  is a single channel fiber optic oxygen meter for WPI’s fiber optic oxygen microsensors.

MicroTip

MicroTip
The MicroTip (WPI #501656) shown aboveis a needle-type (27 ga.) oxygen micro sensor designed for applications where a small tip size of 50µm and a fast response time (t90) of 1s are necessary. The oxygen sensitive sensor tip consists of 140µm fiber tapered to a 50µm tip. The sensor is housed inside a stainless steel needle of 22mm length and 0.4mm diameter. This allows penetration through a septum rubber or similar material. These sensors are ideal for oxygen profiling in sediments and biofilms.

MicroFlow

MicroFlow
The MicroFlow fiber optic oxygen sensor (WPI #501657) shown above is a miniaturized fiber optic chemical sensor optimized for fast response time (t90 < 1s in gases, < 5s in liquids). The tiny probe has a tip size of 50µm and is integrated in a T-shape flow cell for easy connection via Luer-Lock adapters to external tubings. Liquids (like water or blood) can be pumped through the cell.

MicroImplant
MicroImplant
The MicroImplant fiber optic oxygen sensor (WPI #501658) shown above is an implantable probe (IMP) with a tiny probe tip size 50µm, an exposed fiber length of 5mm and a jacket diameter of 900µm. The IMP sensor was successfully implanted in crabs, fishes and soil.

OXY-MINI System

The OXY-MINI  is a single-channel fiber optic oxygen meter for WPI’s fiber optic oxygen minisensors. These sensors are based on 2mm polymer optical fibers and have a length of 2.5m. A wide range of applications is possible with these sensors.
MiniTip
MiniTip
This oxygen dipping probe (WPI #501641) shown above has a tip diameter of 4 mm and consists of a polymer optical fiber, with an oxygen sensitive coating. The MiniTip’s range is 0 to 100%. This robust sensor has a response time (t90) of approximately 40s.
MiniFlow

MiniFlow

The MiniFlow oxygen probe (WPI #501642) shown above is a miniaturized fiber optic chemical sensor integrated in a T-shape flow through cell. The standard T-shape flow cell can be easily connected via Luer-Lock adapters to external tubings. Liquids (e.g., water, blood, etc.) can be pumped through the cell. The sensor has a response time (t90) of approximately 40s and an excellent long-term stability.
MiniFoil
WPI offers the sensor material on a 5mm diameter support disk made of polyester. This material can be glued, for example, inside glass vials and the oxygen concentration can be measured non-invasively and non-destructively from outside through the wall of the flask. A plastic fiber optic cable (WPI #501644) is used to illuminate the sensor foil. The wall of the flask must be transparent/non-fluorescent. Response time (t90) of approximately 50s. The material can be implanted into animal tissues or custom-made housings.

MiniTipMiniFlowMiniSpotMicroTipMicroFlowMicroImplant
Measurement Range
dissolved/gaseous
0-45ppm,
0-100%
0-760mmHg
0-45ppm,
0-100%
0-760mmHg
0-45ppm,
0-100%
0-760mmHg
0-45ppm,
0-100%
0-760mmHg
pt;">0-45ppm,
0-100%
0-760mmHg
0-45ppm,
0-100%
0-760mmHg
Response Time [t90]
dissolved/gaseous
40s
10s
40s
10s
40s
10s
< 2s<br<> < 2s
< 0.5s
< 2s
< 0.5s
Sterilization (EtOH, H2O2)
autoclavable (130°C, 1.5 atm)
Y
N
Y
Y
Y
Y
Y
N
Y
Y
Y
Y
Drift (100,000 datapoints, 20°C) < 0.1% < 0.1% < 0.1% < 0.3% < 0.3% < 0.3%
Accuracy (20°C) 0.2%
Resolution (20°C) 2.75 ±0.01ppm, 9.00 ±0.05ppm, 220 ±0.15ppm, 45.0 ±0.25mmHg, 150 ±0.75mmHg, 375 ±2.6mmHg
Temperature Range -10°C to 50°C
Probe Assembly Length 2.5m

 

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