Cell Tester Boast Some Ingenious Features
Physiologic Mechanisms in Cardiac Myocytes and Skeletal Muscle Cells
The revolutionary Cell Tester SI-CTS200 is a new research tool for cellular investigation that can (without any changes) be used for one single living cell, for a small multi-cellular preparation and for single or larger skinned muscle strip preparations. Translational experiments from the single living cells to the intact multi-cellular level can be accomplished. For example, using the Cell Tester, the influence of the connective tissue on muscle function can be distinguished from the clean muscle work for the first time. Conversely, skinning allows a direct comparison between the living cell response and a cell, whereby the subcellular contractile proteins are studied with full experimental access to cell signalling and cellular biochemistry.
The Cell Tester offers:
- Integral microtweezer apparatus that facilitates cellular attachment
- Two integrated piezo manipulators are included
- Unique rotational stage that allows for easy cellular alignment, improved experimental throughput (shown in the image above)
- Ultra-quiet force transducer included
- Linear displacement motor stretches or compresses cells with 25nm precision
- Fits ANY inverted microscope
- Use native cuvette or ANY 35mm glass bottom dish
The Cell Tester provides researchers with the comprehensive ability to investigate and characterize the physiological, bio-mechanical and bio-physical properties of single isolated living cells and extend these findings to the sub- and multi-cellular level.
The elegance of this system is found in its simplicity of design. For example, the rotating cuvette comes with two interchangeable inserts. The first holds any 35mm glass bottom dish (WPI #FD35-100). When coating tweezers or glass rods with a biocompatible adhesive, insert a FluoroDish into the holder and place it in the rotating cuvette. When finished, remove the insert and dispose of the FluoroDish. Then, insert the native cuvette insert containing the live cells.
The cuvette has two interchangeable inserts. The second image above shows the 35mm fluoroDish insert in the cuvette holder. It is a disposable dish that can be used for coating parts with adhesive. Your live cells can remain in the native insert until you are ready.
Consider the pair of precision piezo manipulators which can be programmed to run precise routines. These manipulators come with an intuitive, ergonomic remote controller. The main display shows the current position information in micrometers, speed setting, id of the micromanipulator that is operated and the battery status. One of the most ingenious features of these manipulators is the back flip mode (shown below). When you need to change tissue mounts on the SI-KG force transducer or the nanomotor, this back flip mode simplifies the process.
The manipulators tilt up and fold back to facilitate system setup and coating with adhesive. There are two programmable memory positions (home and target) for easy exchange of the 35mm dishes, providing access to the bath so you can add the live cells. The combination of these features enables high experimental throughput.
The compact micromanipulators and the Cell Tester's universal design allows this entire system to fit on ANY inverted microscope.
The electronics controlls for the Cell Tester, including the Nanomotor position controller, the SI-BAM21LCB amplifier, the SI-AOSUB anti-oscillation unit and the SI-TCM2 2-channel temperature control module, all fit in a single Signal Conditioning Amplifier System (shown below). CLICK ON THE IMAGE TO SEE A BIGGER PICTURE. This puts all the controls in a single, rack-mountable chassis and ensures that all the controls work together as designed for the precision control you require.
Click thumbnail for a larger image. All the Cell Tester electronics are mounted in a single chassis and connected internally to eliminate extraneous wiring and reduce the amount of space that individual control units would require.
For more information on the new Cell Tester 200, contact WPI to speak with one of our staff muscle physiologists by email at email@example.com or by telephone at 866.606.1974.