APP NOTE: Microinjection Setup 101

When it comes to setting up microinjection systems, the options appear endless. The pictures below give some broad suggestions on how you might set up your own system. Keep in mind that many parts are interchangeable depending on your needs or preferences.

In general, you will need a stereo microscope on a stand, a light source, one or two micromanipulators with stands, and one or two injection systems. The following images show various setups for microinjection, and all the WPI part numbers are included for easy reference.

Remember, when you set up your own system, choose the parts that fit your needs. For example:

• M10 or the M9 magnetic base could be used.
• PZMIV stereo microscope could be used instead of the PZMIII stereomicroscope.
• M3301 or the KITE micromanipulators can be used, and these micromanipulators can be placed on either side. (Keep in mind, though, if you wanted to use a KITE micromanipulator on the right side of the setup below, you would order a KITE-R (right hand), or if you want an M3301 on the left side, you would order an M3301-L.)
• 5479 or 5052 magnetic bases are virtually interchangeable.
• One or two Nanoliter 2010 nanoliter injection systems, one or two UMPIII systems, or one Nanoliter 2010 and one UMPIII micro-injection system may be used, as desired.

ONE NANOLITER/ONE UMP3-1

ONE NANOLITER

ONE NANOLITER/M3301

ONE NANOLITER/KITE

ONE UMP3-1

See Selection