Biosensing
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June 09, 2020
[by Nikki Scafa]
When using a TBR Free Radical Analyzer, accurate measurements of an analyte requires an accurate calibration. The calibration of nitric oxide, hydrogen peroxide and hydrogen sulfide sensors is similar. WPI oxygen and glucose sensors require different methods which will not be discussed here.
Before we begin:
- Make sure you have the following items.
- Your sensor must be polarized for the appropriate length of time.
- Your solutions are prepared as described in the manual. (NOTE: Here we will show an item checklist slide (below) before continuing the video.)
...more
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February 25, 2020
Nitric oxide (NO) is an essential signaling molecule and is known to play a significant role in a multitude of physiological systems including the central nervous system (CNS), the cardiovascular system, the gastrointestinal tract, the immune system, and the renal system. 1-5 However, being highly reactive, detection and quantification of NO is very difficult.6,7 It requires a sensor that is sensitive, selective to NO, and easy to calibrate.
WPI’s Free Radical Analyzer (4-channel TBR4100 and single-channel TBR1025) and the LabTrax Data Acquisition System with the options of using nano NO sensors, micro NO sensors, ...more
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October 10, 2014
World Precision Instruments chemist Nikki Scafa demonstrates how to calibrate your ISO-OXY-2 or OXELP oxygen sensor with the TBR4100 free radical analyzer. For more information on biosensors, see www.wpiinc.com/biosensors.
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April 30, 2013
A New Generation of Fiber Optic Oxygen Sensors Based on Luminescence Lifetime
Oxygen measurement is simpler than ever. Just stick a disposable, oxygen-sensitive "spot" to the inside of a flask, beaker, test tube or bottle, and fill the container
with the solution to be tested. Then, on the outside of the glass container, hold the fiber optic wand close to the spot to take a reading. As the spot reacts with oxygen, it gives off light, which is measured with the fiber optic wand. This ingenious system is highly accurate and affordable. Two different units (which use the same operating principles are available: OXY-MINI and OXY-MICRO. The OXY-MINI system is optimized for process control and biotechnology applications...more
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April 29, 2013
Download a PDF version of the Biosensor Specifications Sheet here.
WPI offers a range of biosensors for monitoring nitric oxide, hydrogen peroxide, oxygen and hydrogen sulfide. Specifications for these sensors are detailed below.
- The macrosensors are 2mm "wet" sensors. These sensors are installed in a metal "sleeve" that looks like a tiny soda straw.The sleeve has a gas permeable membrane at the tip, and it is filled with an electrolyte. When it is immersed in a solution, the gas in solution (for example, nitric oxide) diffuses through the membrane, and the sensor measures it.
- The microsensors are "dry" sensors. Most microsensors monitor nitric oxide, and there is also a hydrogen peroxide microsensor...more
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April 24, 2013
In this video, WPI Chemist Nikki Scafa demonstrates how to make a 0.1M solution of CuCl2 for calibrating WPI NO microsensors.
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April 24, 2013
In this video, WPI Chemist Nikki Scafa demonstrates how to make a PBS buffer solution for use with WPI microsensors.
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April 24, 2013
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April 24, 2013