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- Microinjection processes use either metal microinjection needles or glass micropipettes to inject small liquid volumes. For example, genetic material may be inserted into a living cell, a drug introduced into an eye or brain, or fluid injected into a muscle. Typically, microinjection is performed under a microscope. A stereotaxic frame setup may be required.
Recently, WPI introduced its customizable Microinjection System with everything you need to get started. We can help you customize your system with many options and microinjection system accessories. The basic system includes:
Microinjection pump like the PV820 Pneumatic PicoPump
LED lighted microscope base with a PZMIII Stereo Microscope and an articulating mirror
WPI offers a variety of pumps along with special syringes, stereotaxic frames, glass capillaries and needles. The setup you choose depends on the size of your microinjection aliquots, the volume to be injected and the size of needle or glass tip you choose. In addition, we offer an electroporator for transfection procedures.
Whether you are working with Danio rerio (zebrafish), Xenopus, Drosophila or Caenorhabditis elegans, we can help you customize a microinjection system for your application.
Our free radical analyzers are used in biosensing experiments to detect oxygen, nitric oxide, carbon dioxide, hydrogen peroxide, hydrogen sulfide and glucose, using WPI ion-species specific biosensors. Our TBR series biosensing analyzers can be set to monitor the specific species of interest, and the 4-channel TBR4100 can monitor four biosensors simultaneously. While the TBR biosensing device is commonly used as a nitric oxide analyzer, it is perfect for real-time detection and measurement of a variety of redox-reactive species using the electrochemical (amperometric) detection principle. This series of optically isolated biosensing analyzers has ultra low noise and independently operated channels
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January 24, 2020Microinjection is the process of transferring genetic materials into a living cell using glass micropipettes or metal microinjection needles. Glass micropipettes can be of various sizes with tip diameters ranging from 0.1 to 10 µm. DNA or RNA is injected directly into the cell’s nucleus. Microinjection has been successfully used with large frog eggs, mammalian cells, mammalian embryos, plants and tissues. Microinjection has been expensive, can be a slow process and requires skilled personnel, but new technologies are making it even more reliable, repeatable and affordable. Pronuclear injection, inserting DNA or RNA into the nucleus of a fertilized oocyte to create transgenic organisms, lets researchers study the role of particular genes. This horizontal gene transfer can insert genetic material from the same species or a
December 20, 2019The purpose of this article is to describe a new cleaning procedure for thoroughly cleaning WPI flowcells, including Liquid Waveguide Capillary Cells (LWCCs), UltraPath flowcells and optical cuvettes. The image (right) shows a 3000 series LWCC with a MiniStar pump and the LWCC injection system placed on top of it. Preparation of Chemicals All chemical reagents should be of at least ACS-Grade, preferably HPLC-Grade. This procedure involves the use of caustic and flammable reagents. Consult the manufacturer’s MSDS for necessary safety precautions. Cleaning Solution #1: 0.5M Potassium Hydroxide in 100% Ethanol (e.g.: 7 g KOH in 250mL EtOH). After thoroughly mixing, filter the solution through a 20µm pore size filter. Cleaning Solution #2: 100% Methanol, HPLC grade Cleaning So
November 11, 2019OEM Optical Fiber Manufacture - Your OEM Partner for Qualified Fiber, Assemblies, Probes and Flow CellsWPI's Photonics Center of Excellence in Friedberg, Germany provides a full range of optical components and optical sensing solutions, including proprietary multimode silica qualified fiber, optical assemblies and sub-assemblies serving