NOW OFFERING A UV ENHANCED VERSION" – The SPT-2
SpectroPipetter™ is the smallest and easiest to use microliter spectroscopic sample cell on the market. With its especially low sample volume of only 2 microliters, the SpectroPipetter is ideally suited for protein and DNA analysis. In its outward design, the SpectroPipetter resembles a standard laboratory pipette. Fluid is taken up and expelled by means of a manual plunger, just as in a standard pipette. The plunger is made of an optical fiber bundle, protected by stainless steel tubing. In addition to moving the sample in and out of the sample chamber, it delivers and collects the light used for absorbance measurements. This design allows easy, quick and accurate spectroscopic measurements of small sample volumes. Two fiber optic cables are used to connect the SpectroPipetter to a spectrophotometer. The launch cable is used to couple light from a light source to the sample chamber, which is designed using waveguide principles. The return guides the light from the sample chamber to a spectrophotometer. The SpectroPipetter is ideally suited for measurements with WPI's TIDAS II spectrophotometer system. Further, modular sample systems can be assembled using WPI’s PDA (TIDAS-I) or CCD (SpectraUSB) based spectrometer modules and WPI’s range of UV/VIS light sources (D2H, D2Lite™, and FO-6000). We recommend the SPT-2 for TIDAS-2 and TIDAS-1-based systems and the SPT for SpectraUSB-based systems.
To exemplify the performance of the SpectroPipetter, Bovine Serum Albumin (BSA) dissolved in a phosphate buffer BSA has been analyzed. Absorbance spectra and a calibration curve are shown. The absorbance spectra were acquired with a TIDAS II (TIDAS-BASIC). Each spectra has been obtained from 30 to 40 µl samples stored in 0.1 mL centrifuge vials.
In addition to the standard accessories, the optional Ultrasonic Cleaning Kit is highly recommended for keeping the sample chamber clean and for removing any residual sample fluid or dye from the sample cell. The kit contains an ultrasonic cleaning bath for the tip module, a special stand for placing the SpectroPipetter in the ultrasonic bath and a bottle of concentrated cleaning solution.
SpectroPipetter Specifications
SPT SPT-2
WAVELENGTH RANGE (with TIDAS II) 230 - 850 nm 230 - 850 nm
SAMPLE VOLUME 2 microliter 2 microliter
LIGHT PATH LENGTH OF CELL 1.0 cm 1.0 cm
TEMPERATURE RANGE 4 to 99 °C 4 to 99 °C
OPTICAL FIBER LENGTH 1.3 m 1.3 m
CORE DIAMETER OF LAUNCH FIBER(S) 400 µm 200 µm bundle (3)
CORE DIAMETER OF RETURN FIBER 100 µm 200 µm
SPT SpectroPipetter
SPT-2 SpectroPipetter-II — Optimal UV Efficiency
SPT-UCK SpectroPipetter & Ultrasonic Cleaning Kit
SPT-2-UCK SpectroPipetter-II & Ultrasonic Cleaning Kit
OPTIONAL ACCESSORIES
15807 Cleaning Solution Concentrate (125 mL)
UCK Ultrasonic Cleaning Kit
References
S.X. Liu, J.P. Fabisiak, V.A. Tyurin, G.G. Borisenko, B.R. Pitt, J.S. Lazo, V.E. Kagan, “Reconstitution of Apo-Superoxide Dismutase by Nitric Oxide-Induced Copper Transfer from Metallothioneins”, Chemical Research in Toxicology, 2000.

S. Aleman, et al., “Long-term effects of antiretroviral combination therapy on HIV type 1 DNA levels” (1999) Aids Research And Human Retroviruses 15: 14.

T.M. Redmond, S. Yu, E. Lee, D. Bok, D. Hamasaki, N. Chen, P. Goletz, J.X. Ma, R.K. Crouch, K. Pfeifer, “Rpe65 is necessary for production of 11-cis-vitamin A in the retinal visual cycle”, Nature Genetics, vol. 20, (1998), 344-351.

U.S. Patents 5,416,879 -- 2,284,904B -- 5,507,447