Pneumatic PicoPumps

Pressure-pulsed micro injector for volumes ranging from picoliters to milliliters
  • Excellent for focal drug delivery
  • Simplify intracellular injection
  • Regulated air pressures for securing cells and injecting with fluid
  • Injected volumes range from picoliters to milliliters
  • Positive pressure for high-pressure ejection, suction for supporting the cell or filling the pipette from the tip
  • Negative pressure port maintains a low positive “holding” pressure to the injecting pipette between injection pulses preventing fluid uptake through capillary action or diffusion.
  • Adjust timing, ejection pressure, holding pressure, and suction independently
  • Injection pressure is controlled by a 20 turn regulator on the front panel.
  • Built-in timing circuit allows precise control of the amount of time that the injection pressure is applied to the output port. Time intervals can range from 10 seconds down to 10 ms or less, depending on the eject pressure setting.
  • The injection pressure interval can be triggered manually on the front panel, by footswitch, or by computer controlled TTL pulse.
  • A 5-volt monitor output provides a logic-level pulse for your computer or other monitoring device.
    PV830
    Eject Pressure, Hold Pressure, and Vacuum are all available, controlled by separate regulators on the front panel.
    Eject Pressure supplies a high-pressure pulse for injecting fluid.
    Hold Pressure, which is not sufficient to cause fluid ejection, is used to prevent back filling of the pipette by capillary action or diffusion when the solenoid is inactive. Pressure in the injection pipette is automatically switched between Eject and Hold Pressure by a precision timing circuit that controls a solenoid valve.
    Eject Pressure is controlled by the range switch and 20-turn regulator knob on the front panel. Vacuum is used to fill pipettes from the tip or to secure a floating cell during microinjection.
    Vacuum is regulated the same way, by a 20-turn knob on the front panel. Vacuum may be switched from regulated vacuum to atmosphere by using the pneumatic toggle on the front panel. Ejection or suction can be timed manually, using the Start button or a footswitch (optional), or timing can be preset on the front panel by using the Range switch and Period knob, or by computer.

    		 PV820
     Eject Pressure and Hold Pressure are available on this unit.
    Hold Pressure is used to maintain a low pressure in the pipette between injections to prevent unwanted fluid uptake by capillary action or diffusion.
    A precision timing circuit switches from Eject Pressure to Hold Pressure automatically, once timing has been set.
    Add vacuum externally
    Although regulated vacuum is not provided in this model, suction can be provided by connecting a vacuum source to the vacuum port on the rear panel. Suction is then available through the pressure ports. Ejection/suction can be timed manually, using the Start button or a footswitch (optional).
    Timing can also be preset by using the Range switch and Period knob on the front panel, or by computer.


    PicoNozzle Kit (5430-ALL) included with both the PV820 & PV830 allows all sizes of glass micropipettes to be securely mounted in micropositioners for stable axial air delivery. Because air enters the pipette axially, lateral whipping during injection is eliminated.
    PicoNozzleKit
    PicoNozzle Kit 5430-XX (see below) - Available as an accessory, choose the kit specifically designed for the size of glass capillaries used in your application.
    PicoNozzleOne


    		References
    PV820
    Morishima, M, et al. "Recurrent Connection Patterns of Corticostriatal Pyramidal Cells in Frontal Cortex" (2006) The Journal of Neuroscience 26(16)4394-4405
    Antonsen, B, et al. "The Retrograde Spread of Synaptic Potentials and Recruitment of Presynaptic Inputs" (2005) The Journal of Neuroscience 25(12):3086-3094
    He, D, et al. "Prestin and the Dynamic Stiffness of Cochlear Outer Hair Cells" (2003) The Journal of Neuroscience 23(27):9089-9096

    PV830
    Griffioen, K,     et al. "Purinergic P2X Receptors Mediate Excitatory Transmission to Cardiac Vagal Neurons in the Nucleus Ambiguus After Hypoxia" (2007) Hypertension 50:75-81
    Wang, X, et al. "Ketamine Inhibits Inspiratory-evoked y-Aminobutyric Acid and Glycine Neurotransmission to Cardiac Vagal Neurons in the Nucleus Ambiguus" (2005) Anesthesiology 103:353-9
    Deveau, J, et al. "An improved method for contructing and selectively silanizing double-barreled, neutral liquid-carrier, ion-selective electrodes" (2005) Bio Proced. Online 7(1):31-40

    Using PV830 for holding and injection of cells
    view application sample

    For a complete list of pre-pulled micropipettes, see µTips,
    or contact us with your special requirements.

    Request a quote


    Saitow, F, et al. "Beta-Adrenoceptor-mediated long-term up-regulation of the release machinery at rat cerebellar GABAergic synapses" (2005) J Physiol 565.2:487-502
    Wang, J,     et al. "Developmental Effects of Ectopic Expression of the Glucocorticoid Receptor DNA Binding Domain Are Alleviated by an Amino Acid Substitution That Interferes with Homeodomain Binding" (1999) Molecular and Cellular Biology 7106-7122
    Gutierrez, A, et al. "Activation of a Ca2+-permeable cation channel by two different inducers of apoptosis in a human prostatic cancer cell line" (1999) J. Physiol. 517:95-107
    Gomez-Viquez, L, et al. "SERCA Pump Optimizes Ca2+ Release by a Mechanism Independent of Store Filling in Smooth Muscle Cells" (2003) Biophysical Journal 85:370-380
    PV820 PicoPump w/ hold pressure
    PV830 PicoPump w/ hold pressure and vacuum
    Specify line voltage. All PicoPumps require external vacuum source -- see below
    Each PicoPump is supplied with one PicoNozzle 2 Kit (5430-ALL - package of 2), for glass ODs of 1.0, 1.2, 1.5, or 1.65 mm, plus tubing to connect the holders to the pressure and vacuum ports as applicable
       
    Optional Acessories

    3260

    		 Foot Switch
    2932 Rack Mount Kit, 31/2-in. high (PV820)
    2933 Rack Mount Kit, 51/4-in. high (PV830)
    5430-ALL PicoNozzle (V.2) Kit (for glass ODs of 1.0, 1.2, 1.5, or 1.65 mm) plus tubing
    5430-10 PicoNozzle Kit (MPH6S for 1.0 mm pipette & 5-ft tubing assembly)
    5430-12 PicoNozzle Kit (MPH6S for 1.2 mm pipette & 5-ft tubing assembly)
    5430-15 PicoNozzle Kit (MPH6S for 1.5 mm pipette & 5-ft tubing assembly)
    5430-20 PicoNozzle Kit (MPH6S for 2.0 mm pipette & 5-ft tubing assembly)
    MPH6S Micropipette Holder (specify 1.0, 1.2, 1.5 or 2.0 mm)
    MPH6R Micropipette Holder (specify 1.0, 1.2, 1.5 or 2.0 mm)
    3316 Replacement Input Kit
    LV140-Y Vacuum Pump (120V only)


    PV800 SERIES SPECIFICATIONS

    		PV820 PV830
    PRESSURE
    Pressure Input 0 to 150 psi 0 to 150 psi
    Pressure Output 0.3 to 90 psi * 0.3 to 90 psi
    Lowest Regulated Pressure 12 in. water * 12 in. water
    Regulator Accuracy 0.1% (20-turn dial) * 0.1% (20-turn dial) *
    Regulator Repeatability 0.05 psi * 0.05 psi *
    Gauge Accuracy 3% at full scale * 3% at full scale *
    Input Connector Quick Connect (1/4 in. OD Tubing) Quick Connect (1/4 in. OD Tubing)
    Output Connector Barbed (1/16 in. ID Tubing) Barbed (1/16 in. ID Tubing)
    Control Solenoid Solenoid
    Vent To holding pressure or vacuumby manual control
    * Both Hold and Eject Pressures * Both Hold and Eject Pressures    		 To vacuum, holding pressure or atmosphere
    VACUUM
    Vacuum Input 0 to 30.0 in. Hg 0 to 30.0 in. Hg
    Vacuum Output Unregulated 0.2 to 29.9 in. Hg
    Lowest Regulated Vacuum Unregulated 3 in. water
    Regulator Accuracy Unregulated 0.1% (20-turn dial)
    Regulator Repeatability Unregulated 0.03 in. Hg
    Gauge Accuracy None 3% at full scale
    Input Connector Quick Connect (1/4 in. OD Tubing) and Barbed (3/16 in. ID Tubing) Quick Connect (1/4 in. OD Tubing) and Barbed (3/16 in. ID Tubing)
    Output Connector Barbed (1/16 in. ID Tubing) Barbed (1/16 in. ID Tubing)
    Control Manual Manual
    Vent Atmosphere Atmosphere
    CONNECTIONS INCLUDED
    Input Kit 12 ft nylon tubing (0.25 in. OD, 1000 psi), two 1/4-in. OD adapter, one female NPT adapter
    Output Kit Two PicoNozzle assemblies, each consisting of one MPH6S pipette holder, 60-in. of PVC tubing (200 psi), and a luer-fitted aluminum handle.
    TIMING
    Duration Modes Gated (by input signal) or Timed (by internal clock)
    Pulse Initiation Manual, External Input or Foot Switch (optional)
    Pulse Width 10 ms to 10 s in Timed Mode (10-turn dial). Lower limit depends upon pressure setting. At 0 psi the minimum pulse width is nominally 8 ms. At 100 psi the minimum pulse width is nominally 3 ms.
    Accuracy 0.1% at full scale
    External Input +5 V TTL-compatible (BNC)
    Monitor Output +5 V TTL-compatible (BNC)
    PHYSICAL SPECIFICATIONS
    Power 95-135 V or 220-240 V, 50/60 Hz 95-135 V or 220-240 V, 50/60 Hz
    Dimensions 17 x 3.5 x 9.5 in. (43 x 9 x 24 cm) 17 x 5.25 x 9.5 in. (43 x 13 x 24 cm)
    Shipping Weight 11 lb (5 kg) 14 lb (6.3 kg)