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NanoFil™ Sub-microliter injection system for small animal research |
| Application Kits |
| These kits are specially designed for eye research for injecting retinal pigment epithelium (RPE) and intraocular (IO) in addition to brain injection in mice. They need to be used with a NanoFil syringe and UMP2 to achieve accurate, repetitive, and oil free injection in the submicroliter range. Each kit includes two pieces of Silflex tubing (one for a spare), a holder assembly, spare gaskets, and an assortment of four tips - blunt for the RPE kit and beveled tips for the IO kit. Each kit comes with one each of 33, 34, 35 and 36 gauge tips so that first time users can find the best size for their application. The Silflex tubing is the most critical component of the kit. This 35 cm long, flexible tubing has a very precise outer diameter for airtight fitting with the syringe. It also has a small inner diameter for minimum dead volume, and is very durable when handled correctly. The SilFlex is coupled to the injection tip with a mechanism similar to that of the NanoFil. The dead volume of the entire kit (including the tubing) is less than 3 microliters. All of the components in the kit are constructed of inert, solvent resistant, and autoclaveable materials for easy cleaning after viral injection. |
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| SilFlex tubing and holder: The needle is mounted on a small plastic holder that is connected to the NanoFil by a 35 cm length of flexible tubing. The NanoFil is mounted on the UMP II pump. This configuration allows the user to hold the animal in one hand and insert the needle with the other. When the needle reaches the desired location, activate the pump using the footswitch and the pre-programmed injection volume will be delivered. This configuration gives a nanoliter level of accuracy and reproducibility. It is best suited for applications such as the RPE and IO injection. |
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Installed on WPI’s UMP-II microsyringe pump: This will allow the user to achieve nanoliter resolution and reproducibility. For neural system injection, mount the UMPII on a stereotaxic frame. |
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Direct injection by hand: This is the simplest and most economical way to inject. Any of our tips can be inserted directly into the NanoFil syringe. Even the SilFlex tubing can be inserted to switch from hand injection to the other methods listed below. The limitation of this method is the difficulty achieving sub microliter resolution.
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