With the wide use of transgenic mice in studies of this kind, the need for tissue support mechanisms capable of handling the extremely small size of vessels and muscle strips harvested from small animals is paramount. WPI offers five micro-tissue holders for use with micro tissue strips, micro vessels and other tissues where the force measurements are less than 10 g.
Micro tissue holders are designed to be used with the 2 mL and 5 mL volume tissue baths and FORT 25 transducer. Microtissue Holders MH1 and MHS1 are designed for muscle strips as small as 0.5 mm in width and 5 mm in length. The muscle strip is held between two miniature vessel clips with a clamping pressure of 60 grams. These holders are most suitable for measuring isometric force of less than 5-10 grams. MHS1 differs from MH1 only in that it has an in-built stimulation circuit and electrodes.
Micro vessel holders MV100, MVS100 and MVS200 require a tissue bath larger than 5 mL and the use of FORT 25 or comparable transducer. These holders are designed for studying tension in vascular rings. When used with the micrometer driven tensioner/positioner they can also be used for measuring the lumen diameter of vessel preparations. The MV100 and MVS100 can be used for micro vessel preparations as small as 500-600 microns (ID). MVS200 is designed for larger vessels (> 2 mm ID). MVS100 and MVS200 feature specially designed spiral stimulation electrodes fabricated from 250-micron diameter platinum wire. The two-and-a-half turns of each spiral electrode span appropriately 4-5 mm. The positions of the spiral electrodes can be adjusted with respect to the tissue placement, a feature that is very useful in controlling stimulus intensity. |
| YYYY |
Part Number |
Description |
| G |
503201 |
Glass Hook |
| V |
47040 |
Vascular Holder |
| VS |
47050 |
Vascular Holder with Stimulation |
| AS |
47030 |
Atria Holder with Stimulation |
| BS |
47060 |
Blank Holder with Simulation |
| MV |
47110 |
Mini Vessel Holder |
| MVS1 |
47120 |
Mini Vessel Holder with Stimulation, Size 1 |
| MVS2 |
47130 |
Mini Vessel Holder with Stimulation, Size 2 |
| MH1 |
47070 |
Mini Tissue Strip Holder |
| MHS1 |
47075 |
Mini Strip-tissue Holder with Stimulation |
|
