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The devices that actually hold tissue for experimentation in a tissue bath are critical to success. They need to be robust, appropriately sized and well thought out in terms of the attachment to the tissue. Since many different sizes and types of tissue can be used in the study of smooth muscle, variety is also critical. WPI offers 10 different application specific tissue support devices.
Five types of tissue holders are available for 10-50 mL organ baths and five types of Miniature Tissue Holders are available for smaller tissue preparations.
In many applications it is desireable to deliver electrical stimulation to the tissue. WPI offers a series of tissue holders with built in stimulation electrodes. Connection to the stimulation device is accomplished with low mass connectors and wire. These ultimately connect to BNC connectors mounted in the stabilizing par of the frame. Connections to isolators or stimulators can then be made with standard coaxial cables.
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Glass Hook (G)
The glass hook is a universal design utilizing made from a glass rod terminating in an S-shaped glass hook. Stainless steel hooks or fine wire hangers are used to secure the tissue between the glass hooh and the force transducer. The glass rod has a diameter 6.5mm and the hook is 2.5 mm away from the side of the glass rod. |
AS Holder (#47030)
The AS holder is used for mounting atrial muscle preparations. This holder is equipped with stimulation electrodes. The tissue is mounted onto the two S-shaped hooks (made from 301/2 gauge needles), The S-hooks measure 2 mm in diameter on both ends with a sharp tip on one end where tissue is pieced. The two point stimulation platinum (Pt) electrodes on the holder are 2.54 mm (0.1in.) apart. |
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V Holder (#47040)
The V holder is used to mount vascular rings. The bottom hook consists of a U-shaped wire. The top hook is an L-shaped wire. The tissue is mounted as shown. The hooks are made from 0.48 mm (0.019 in.) stainless steel wire. The U-shaped hook measures 6.35 mm (0.25 in.) in base. |
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Tissue Bath
Tissue Holders
Micro Tissue Holders
Transducers and Amplifiers
Data Systems
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VS Holder (#47050) V Holder with Stimulation
The VS holder uses the same tissue-mounting mechanism as the V holder, but has two 5 mm flexible platinum stimulation electrodes, which can be positioned around the tissue. The VS holder can only be used in the 25mL or 50mL baths. |
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B Holder with Stimulation (BS) The B holder is designed for custom-made hooks. The bottom tissue hook is secured with a nylon screw. Stainless steel wire (0.56 mm or 0.022 in.) provided in the startup kit can be used to make the top hook that connects to the strain gauge. Platinum electrodes for point stimulation are identical to those on the AS holder. |
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