Tissue Bath |
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 WPI offers five different tissue baths: 2 mL, 5 mL, 10 mL, 25 mL, and 50 mL. This range is sufficient to cover a broad variety of applications including those involving smaller vessels and tissues from mice or other small animals.Each tissue bath has 6 ports. The baths are jacketed to maintain the temperature of the perfusate in the center well. The jacket has in inlet and an outlet. A separate inlet with a ceramic frit is used to aerate the perfusate. The frit is designed to produce smaller bubbles that are less prone to strike the tissue or supporting hangers hard enough to cause a perturbation in the force signal. The bath is also provided with a drain port at the bottom of the chamber, an overflow port and a fill port positioned at the top. The overflow insures that perfusate delivered into the bath will not overflow the chamber and contaminate the work surface. It is also critical for automated fill and drain operations using the optional valve bank and valve controller. |
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Perfusion and Manifolds |
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 Automated Filling and Draining In the standard configuration, both the filling and draining are fed by gravity. Optional automated control of filling and draining is available for four-bath configurations. This option provides efficiency and statistical stability in multi-channel experiments by reducing human interaction. In this configuration, each channel has a solenoid valve control in the filling connection and a solenoid valve control in the draining connection. While filling is still fed by gravity, draining is enhanced with a built-in pump. The solenoid valves are controlled with SVC108 controller, which has 9-pin D-Sub connector for external TTL control signals. The 4-channel auto filling and draining system 472-4AFD includes two 4-channel solenoid valves and one 8-channel solenoid valve controller. Aeration A 6-channel aeration manifold, designed to regulate the flow of O2/CO2 mixtures, is mounted on the top of the center stainless steel post. Four regulators (needle valves) are used to regulate the flow of O2/CO2 to each individual tissue bath. The remaining two regulators are used to supply O2/CO2 to the buffer reservoir and dissection dish. The buffer reservoir holds warmed, oxygenated buffer solution to fill the tissue baths. The tissue baths and the buffer reservoir are jacketed and require a heating and circulating pump to maintain the desired temperature (e.g., WPI #500787). |
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Positioners |
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 Micrometer Positioner/TensionerThe optional Micrometer Transducer Positioner (#47700) can be used to precisely tension tissues and keep track of the exact position or extension of the tissue. The Y axis position can actually be read out from the positioner and fed to a channel of the data system so length and tension can be simultaneously plotted. The user should specify P (for transducer position) or M (micrometer head) in the name. |
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 Classic leadscrew driven positioner/tensionerThe Transducer Positioner (#502198) holds any transducer firmly on a stand or rack. The transducer carrier is slotted to accommodate transducers that have their connecting cable exiting from the handle. The device allows the vertical position of the transducer to be easily adjusted with excellent resolution. The compact design keeps the distance between transducer and mounting pole to a minimum. This results in better stability and less vibration pickup. A release button on the positioner allows it to slide freely up or down for quick coarse adjustment. Fine adjustment is achieved by turning the knob on the top. The positioner can be mounted on a pole with a diameter of up to 12.7 mm (1/2-in.). Transducer handles from 7 mm to 11 mm can be mounted on the holder block. Sensor cables can be fed through the open slot in the clamp so that transducers with both ends larger than 11 mm can still be mounted. Total end-to-end travel is 85 mm. |
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Myobath II |
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