|
|
|
NITRIC OXIDE SENSOR GUIDE
|
| APPLICATIONS |
in vivo
|
- Cell cultures
- NO-2, NO-3
|
Cell cultures
|
Tissue Bath
|
Tissue Bath |
Microvessels |
Microvessels |
Single Cell
|
| SENSOR |
ISO-NOPF
|
ISO-NOPMC
|
ISO-NOP
|
ISO-NOP30L
|
ISO-NOP70L |
ISO-NOP30
|
ISO-NOP007 |
ISO-NOPNM
|
| SENSOR DIAMETER |
100 & 200 µm |
4 mm
|
2 mm
|
30 micron
|
70 micron
|
30 micron
|
7 micron
|
100 nm
|
| RESPONSE TIME |
< 5 sec
|
< 5 sec
|
< 5 sec
|
< 3 sec
|
< 3 sec
|
< 3 sec
|
< 3 sec
|
< 3 sec
|
| LOWEST DETECTION LIMIT |
0.2 nM
|
0.3 nM
|
1 nM
|
1 nM
|
1 nM
|
1 nM
|
0.5 nM
|
0.5 nM
|
| TEMPERATURE SENSITIVITY |
some
|
some
|
yes
|
yes
|
yes
|
yes
|
yes
|
some
|
| DRIFT |
none
|
none
|
none
|
none
|
none
|
none
|
none
|
none
|
| SENSITIVITY |
10 pA/nM
|
15 pA/nM
|
2 pA/nM
|
1.4 pA/nM
|
1.4 pA/nM
|
1.4 pA/nM
|
1 pA/nM
|
0.5 pA/nM
|
| PHYSIOLOGICAL INTERFERENCE |
none
|
none
|
none
|
none
|
none
|
none
|
none
|
none
|
|
-
|
|
|
|
|
NITRIC OXIDE SENSORS |
| ISO-NOPF |
A unique flexible NO sensor! Designed for arteries, microvessels, in vivo applications, and similar applications.
ISO-NOPF electrodes are the newest addition to WPI’s nitric oxide sensor family and are available in 100 µm and 200 µm diameters.The figure (to the right) illustrates the response of an ISO-NOPF. Utlilzing the latest advances in nano-technology and material science, scientists at WPI’s Sensor Laboratory have created these completely flexible and virtually unbreakable NO sensors. The new sensors are based on a composite graphite NO-sensing element combined with a reference electrode. The surface of the sensor is then coated with a unique multi-layered NO-selective membrane |
 |
. |
| ISO-NOP The original nitric oxide probe – ideal for cell cultures, cell suspensions and many other applications |
The ISO-NOP is a popular, robust and high performance sensor encased within a 2 mm diameter disposable stainless steel protective sleeve. The tip of the sleeve is covered with a NO-selective membrane. Replacement membrane sleeves can be purchased separately (WPI #5436) and require an internal electrolyte (WPI #7325).
The ISO-NOP is an excellent general purpose NO sensor.
NO–3 and NO–2 Detection
A simple change in experimental protocol will enable the ISO-NOP to be conveniently used for rapid accurate determination of nitrite (NO–2) and nitrate (NO–3) concentration in samples. Using this method a detection limit for NO–2 or NO–3 as low as 1 nM is routinely possible.
S-nitrosothiol (thionitrite) Detection
Applications describing the use of WPI’s NO sensors for quantitative determination of S-nitrosothiols in biological samples have also been described (Pfeiffer et al. (1998), Zhang, et al. (2002).
|
 |
|
| ISO-NOPMC |
ISO-NOPMC – The world’s first microchip NO sensor, ideal for cell cultures, cell suspension studies, biochemical assay and other similar applications.
The ISO-NOPMC is a unique microchip-based NO electrode. It has a detection limit of less than 0.3 nM (300 pM) NO and is the most sensitive electrochemical NO sensor in the world [Zhang & Broderick, 2000].
The ISO-NOPMC is based on state-of-the-art technology that utilizes a microelectrode wafer deposited on a single silicon chip. (see graphic below) illustrates the sensors response to successive additions of 1.5 nM NO. The ISO-NOPMC is particularly suited for monitoring NO in cell cultures, cell suspensions and any other similar application where ultra-low detection of NO is required. |
|
 |
|
- ISO-NOP30-L
- ISO-NOP70-L
|
The only L-shaped NO sensor on the market. Designed for tissue bath studies.
The ISO-NOP30-L is a unique L-shaped nitric oxide sensor designed specifically for use in tissue bath studies and similar applications (e.g., see WPI’s MYOBATH). The shape of the sensor has been engineered to facilitate placement of the electrode within the lumen of the tissue vessel under study. The ISO-NOP70-L is similar in construction to the ISO-NOP30, but with advantage of having a flexible tip (70 mm diameter).
|
 |
|
ISO-NOP007 & ISO-NOP30 7 and 30 micron sensors with exceptional performance — ideal for tissues and microvessels
|
| The ISO-NOP007 and ISO-NOP30 have recently been improved in design and performance. The ISO-NOP007 has a tip diameter of just 7 microns. The ISO-NOP30 has a tip diameter of 30 microns and is available in two different tip lengths (i.e., ISO-NOP3020 has tip length of 2mm, ISO-NO3005 has tip length of 0.5 mm). The response of the ISO-NOP007 and ISO-NOP30 is linear over a wide dynamic concentration range of NO. The design of both electrodes is based on a single carbon fiber coated with WPI’s NO-selective membrane. A detection limit of approximately 1 nM NO makes these electrodes ideal for use in tissues and microvessels |
|
 |
|
|
| ISO-NOPNM The world’s smallest nitric oxide NanoSensor, designed for measurement of NO at the cellular level |
| The ISO-NOPNM NanoSensor has a tip diameter of just 100 nm (0.1 µm) and a detection limit for NO of less than 0.5 nM — making it indisputably the smallest and most sensitive NO sensor in the world! The ISO-NOPNM is based on a novel design in which an electrochemically 'activated' composite graphite nanofibre is used as the NO-sensing element . The surface of the NanoSensor is then modified using a unique multi-layered NO-selective membrane. The graph illustrates the response of the ISO-NOPNM following successive additions of nanomolar concentrations of NO. The ultra-low noise of the ISO-NOPNM (0.5 pA) enables a detection limit of just 0.5 nM NO. The response time of ISO-NOPNM is less than 3 seconds. |
 |
 |
|
| Nitric Oxide Accessories |
| 5435 |
ISONOP Startup Kit (recommended with first purchase) |
| ISO-NOP |
Replacement 2 mm shielded sensor and cable |
| ISO-NOPF100 |
100 µm Flexible NO Sensor, pkg of 2 |
| ISO-NOPF200 |
200 µm Flexible NO Sensor, pkg of 2 |
| ISO-NOP007 |
7 µm Nitric Oxide Sensor (pkg of 3) |
| ISO-NOPMC |
Microchip Nitric Oxide Sensor (6-month warranty) |
| ISO-NOP3020 |
30 µm Sensor Tips (2 mm length), pkg of 3 (requires #91580) |
| ISO-NOP3005 |
30 µm Sensor Tips (0.5 mm fiber), pkg of 3 (requires #91580) |
| ISO-NOPNM |
100 nm NanoSensor, pkg of 3 (requires cable #91580) |
| ISO-NOP30-L |
L-Shaped NO Sensor, pkg of 3 |
| 5436 |
Replacement Sleeve Kit for 2 mm sensor, pkg of 4 |
| 7325 |
ISO-NO Electrolyte (10 mL) |
| 7521 |
ISO-NO Electrolyte, CO2-insensitive (10 mL) |
| 5399 |
T-Adapter Kit (pkg of 3) for ISO-NOP |
| 7357 |
Nitrite Standard Solution, 1 gram/liter (100 mL) |
| Hydrogen Peroxide Accessories |
| 600011 |
ISO-HPO Startup Kit (recommended with first purchase) |
| ISO-HPO-2 |
2mm Shielded HPO Sensor & Cable |
| ISO-HPO-100 |
100 µm HPO Sensor (requires #91580), pkg of 3 |
| 600012 |
Replacement Sleeve Kit for ISO-HPO-2, pkg of 4 |
| 100042 |
ISO-HPO-2 Electrolyte (10 mL) |
| Oxygen Accessories |
| 5377 ISO-OXY |
Startup Kit (recommended with first purchase) |
| ISO-OXY-2 |
2 mm Shielded Oxygen Sensor & Cable |
| 5378 |
Replacement Electrode Sleeve Kit, pkg of 4 |
| 7326 |
ISO2 Filling Solution (electrolyte) |
| Temperature Accessories |
| ISO-TEMP-2 |
2 mm Platinum RTD Temperature Sensor (requires #91580) |
| General Accessories |
| 91580 |
Microsensor Adapter Cable |
| NOCHM-4 |
Four-Port Closed Chamber |
|
