Duo 773
Dual Microprobe System 2-channel intracellular amplifier
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Headstage — Two gold-plated, epoxy sealed miniature active probes can be positioned directly to the measurement site. Microelectrode holders containing an Ag/AgCl electrochemical half-cells plug directly into the probes. Stray capacitance can be reduced by placing the included driven guard shield over the microelectrode holder at the end of the probe. Capacity Compensation — Channel A can compensate up to 10 pF of electrode shunt capacity and Channel B can compensate up to 50 pF. Tickler Circuit — Assists in cell penetration. The frequency and amplitude of the oscillations may be varied for differences in membrane thickness or cell size. The duration of tickle can be controlled either by using the momentary switch, a foot switch, or by applying a signal to the remote tickler input. Active Filters — Low pass settings on a -40 dB/decade active filter vary the cutoff from 1 to 30 kHz. Either probe or bridge outputs may be selected for filtering. Current Injection — Channel B can eject current through the microelectrode by applying a command signal to the stimulus input connector; the resulting output from the probe will then be a constant current replica of the input signal. Two ranges of current delivery are provided: 50 nA and 500 nA or by an external source. This source can be useful for delivering hyperpolarizing currents to stabilize the cell membrane potential and as a holding current for microiontophoresis. Compliance Alarm — When the electrode voltage exceeds the probe input maximum allowed voltage, an audible over-compliance alarm will sound. Bridge Balance — Subtracts the excess electrode voltage associated with delivering current through the recording micropipette. Electrode resistances up to 1000 M can be balanced in two ranges. The balanced signal is available from x10 or x50 front panel output connectors. Independent Outputs — The Duo773 has an output for each probe independent of gain filtering or balancing. In addition the Duo773 has a 10x and a 50x output for easy integration to most data aquisition programs. Digital Meter — The Duo773 comes complete with a 31/2-digit display for monitoring injection current or the voltages for either probe (single ended or differential). |
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| VIEW A DUO 773 TYPICAL SETUP | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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* Although injected currents are "constant," the maximum current in a given situation will always be limited by the system compliance of 10 V.
**The 712P headstage may be used on either A or B channels, however Current Injection specifications do not apply when used on channel A. The 715P headstage may not be used on the B channel. |
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| See Dri-Refs | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| See Cables and Connectors | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Microelectrode Holders | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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