Nanoliter 2000 Injector 120 V

Injection volumes

WPI™s microprocessor-controlled Nanoliter 2000 uses direct piston displacement. By either pushing the injection button on the control box or pressing on the optional footswitch, a calibrated volume will be smoothly injected. The process is quiet and vibration free. Capillary filling and injection speeds are 23 nL/sec and 46 nL/sec (emptying speeds are 92 nL/sec and 230 nL/sec). Maximum fluid ejection is 5 µL. Each unit comes with sufficient glass to pull at least 300 tips. Glass is 1.14 mm O.D. (nominal) and 0.5mm I.D. By setting the DIP switch, the injection volume can be changed from 2.3 to 69.0 nL in 16 steps. Up to 100 injections may be triggered per filling. Since the volume of a normal Xenopus oocyte is about 500 nL, the instrument has the capability to inject from less than 1% to over 10% of the total volume of the oocyte in one preset step increment.

Included with pump

Included: 1 vial 3.5 in. capillaries (100); 1 vial 7 in. capillaries (100); replacement "O" rings; Allen wrench; MicroFil™ MF34G backfilling needle; and two sample pre-pulled micropipettes.

Smart controller

SYS-MICRO4, an optional microprocessor-based controller, can provide an "intelligent" and easy-to-use interface to up to four Nanoliter Injectors. Operating parameters are set with the membrane key-pad and LCD display. From the key­pad the user can set pump to infusion or withdrawal mode, enter the volume to be infused or withdrawn, and rate of delivery, as well as synchronize the starting and stopping of a combination of Injectors. User parameters can be stored in the device's "non-volatile" memory for instant recall when the unit is powered on. An optional footswitch can be plugged into a connector on the rear of the controller for "hands free" start/stop operation. An RS-232 port on the rear of the controller can be used to connect it to a computer for use with computer control programs.

Troubleshooting Tip 

INJECTION VOLUME	Variable in discreet aliquot volumes
REMOTE CONTROL	Yes
GLASS OD	1.14mm
GLASS ID	0.5mm
STEP	12.7µm/step
INJECTION SPEED: Slow	23nL/sec
INJECTION SPEED: Fast	46nL/sec
FILL SPEED: Slow	23nL/sec
FILL SPEED: Fast	46nL/sec
EMPTY SPEED	92nL/sec
VARIABLE VOLUME RANGE	2.3 - 69.0nL
SMALLEST VOLUME	2.3nL
INJECTIONS PER FILLING, MAX.	100 injections
SHIPPING WEIGHT	3 lb. (1.1 kg)

NANOLITER2000

Chinese Manual

Wilcox & Welch "Interaction between nitric oxide and oxygen radicals in regulation of tubuloglomerular feedback" Acta Physiologica Scandinavica 168. 2000: 119-124

To place an order for this product, please contact the appropriate WPI office or distributor.

Customers from North America can order direct from our corporate office:
World Precision Instruments
175 Sarasota Center Boulevard
Sarasota, FL 34240-9258

• Phone or Fax
  Toll-free: 866-606-1974 Tel: 941-371-1003 Fax: 941-377-5428
Our Customer Service Representatives are available 8am - 6pm EST.
• Online Store
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International customers, please see our office and distributor contact information list.

13142	Footswitch for Nanoliter 2000
15867	Footswitch for Micro4 Controller
300033	Adapter for Micro4
4878	Replacement 3.5-in. glass capillaries (300)
4879	Replacement 7-in. glass capillaries (300)
5340	Spare Parts Kit (includes MicroFil MF34G, displacement piston, five O-ring sets
500299	Pistons, 5-pack
500778	Replacement Nanoliter Injector Universal Adapter
502859	Flared O-Ring Kit
503066	Flared Glass, 100 piece.
SYS-MICRO4	Micro4 Controller, 4-channel
TIP10XV119	Micropipettes for Nanoliter Injector (10)
MF34G-5	MicroFill, 34g (pkg. of 5)

The following images show various setups using the Nanoliter 2000. Keep in mind that parts are interchangeable. For example:

• M10 or the M9 magnetic base could be used.
• PZMTIV microscope could be used instead of the PZMIII.
• M330l or the KITE micromanipulators can be used, and these micromanipulators can be placed on either side. (Keep in mind, though, If you wanted to use a KITE on the right side of the setup below, you would order a KITE-R (right hand), or if you want an M3301 on the left side, you would order an M3301-L.)
• 5479 or 5052 magnetic bases are virtually interchangeable.
• One or two Nanoliters, one or two UMPIII systems, or one Nanoliter and one UMPIII may be used, as desired.

ONE NANOLITER/ONE UMP3-1

ONE NANOLITER

ONE NANOLITER/M3301

  ONE NANOLITER/KITE