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UMP3 includes the pump head only. For controller information, see Micro4.
Picoliter volumes
With its digital controller, UltraMicroPump III can dispense as little as 600 picoliters per incremental advance of the syringe piston (using a 0.5μL syringe). Syringes may be filled externally and then inserted into the pump or filled while mounted in the pump. Fluids injected or withdrawn are held entirely within the micro syringe to maintain a low fluid dead volume.
Positioning
For positioning, the UltraMicroPump III may be attached to any of several WPI micropositioners such as the M3301 (manual), DC3001 (motorized), or any manual stereotaxic manipulator.
Smart Controller
An Integral component in the UMPIII system is a microprocessor-based controller, SYS-MICRO4, which provides an "intelligent" and easy-to-use interface to up to four syringe pumps. Operating parameters are set with the membrane keypad and LCD display. From the keypad the user can select the following functions: set pump to infusion or withdrawal mode, enter the volume to be infused or withdrawn, rate of delivery, and syringe type as well as synchronize the starting and stopping of any combination of syringe pumps. User parameters can be stored in the device's "non-volatile" memory for instant recall when the unit is powered on. An optional footswitch can be plugged into a connector on the rear of the controller for "hands free" start/stop operation. Computer Control - An RS-232 port on the rear of the controller can be used to connect it to a computer for use with computer control programs.
NORMAL MODE
| Total # of Steps |
20,000(63mm travel) |
Minimum Dispensing Volume
|
0.58nL/step (10μL syringe)
|
Linear Motion per Step
|
3.175μm
|
Weight
|
325g (11.5 oz.)
|
Mounting Rod Diameters
|
7.9mm (0.31 in.)
|
Controller Power Requirements
|
2A, 12VDC
|
| Dimensions |
Ų32mm x 190mm (1.3 in. x 7.5 in.)
|
MICROSTEPPING MODE
Precision is increased eight fold.
V. Cherezov, A. Peddi, L. Muthusubramaniam, Y.F. Zheng, M. Caffrey "A robotic system for crystallizing membrane and soluble proteins in lipidic mesophases" Acta Cryst 60. 2004: 1795-1807
Gregory D. Sturbaum, Carrie Reed, Paul J. Hoover, B. Helen Jost, Marilyn M. Marshall, and Charles R. Sterling, "Species-Specific, Nested PCR-Restriction Fragment Length Polymorphism Detection of Single Cryptosporidium parvum Oocysts", Applied and Environmental Microbiology, June 2001, p. 2665-2668, Vol. 67, No. 6
Antje S. Bernd, Makoto Aihara, James D. Lindsey, and Robert N. Weinreb, "Influence of Molecular Weight on Intracameral Dextran Movement to the Posterior Segment of the Mouse Eye", Investigative Ophthalmology and Visual Science. 2004;45:480-484.
Kiyohiko Takayamaa, Takashi Torashimaa, Hajime Horiuchia and Hirokazu Hirai, "Purkinje-cell-preferential transduction by lentiviral vectors with the murine stem cell virus promoter ", Neuroscience Letters, Volume 443, Issue 1, 26 September 2008, Pages 7-11
Torashima, Takashi; Yamada, Nobuaki; Itoh, Masae; Yamamoto, Akitsugu; Hirai, Hirokazu, "Exposure of lentiviral vectors to subneutral pH shifts the tropism from Purkinje cell to Bergmann glia", European Journal of Neuroscience. 24(2):371-380, July 2006.
Takashi Torashimaa, b, d, Shigeo Okoyamac, Tomoyuki Nishizakid and Hirokazu Hirai, "In vivo transduction of murine cerebellar Purkinje cells by HIV-derived lentiviral vectors", Brain Research, Volume 1082, Issue 1, 12 April 2006, Pages 11-22
Shawgo, Rebecca Scheidt, "In vivo activation and biocompatibility of a MEMS microreservoir drug delivery device" , Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2004.
Christiana J. Johnson, Lennart Berglin, Micah A. Chrenek, T.M. Redmond,
Jeffrey H. Boatright, and John M. Nickerson, Molecular Vision, "Technical Brief: Subretinal injection and electroporation into adult mouse eyes" , v.14, 2008.
Aaron M. Jasnow, Donald G. Rainnie, Kimberly A. Maguschak, Jasmeer P. Chhatwal and Kerry J. Ressler, "Construction of Cell-Type Specific Promoter Lentiviruses for Optically Guiding Electrophysiological Recordings and for Targeted Gene Delivery", Methods in Molecular Biology Vol. 515, Viral Applications of Green Fluorescent Protein, Humana Press, 2009; Pp 1-15
To place an order for this product, please contact the appropriate WPI office or distributor.
Customers from North America can order direct from our corporate office:
World Precision Instruments
175 Sarasota Center Boulevard
Sarasota, FL 34240-9258
- Phone or Fax
Toll-free: 866-606-1974 Tel: 941-371-1003 Fax: 941-377-5428
Our Customer Service Representatives are available 8am - 6pm EST.
- Online Store
Many of our products are available for purchase through our online store.
At this time we can only accept orders from the U.S. through the online store.
-
Want instant pricing? Order a free catalog here
International customers, please see our
office and distributor contact information list.
The following images show various setups for microinjection. Keep in mind that parts are interchangeable. For example:
- M10 or the M9 magnetic base could be used.
- PZMIV microscope could be used instead of the PZMIII.
- M330l
or the KITE micromanipulators can be used, and these micromanipulators
can be placed on either side. (Keep in mind, though, If you wanted to
use a KITE on the right side of the setup below, you would order a
KITE-R (right hand), or if you want an M3301 on the left side, you
would order an M3301-L.)
- 5479 or 5052 magnetic bases are virtually interchangeable.
- One or two Nanoliters, one or two UMPIII systems, or one Nanoliter and one UMPIII may be used, as desired.
ONE NANOLITER/ONE UMP3-1
ONE UMP3-1
