Std Muscle Research System with Heated Cuvette/Linear Motor

Click here to view the current Data Sheet.
Click here for information on Force Transducers.

THE PHOTO ABOVE SHOWS THE MKB SYSTEM WITH A MOTOR AND OPTICAL CUVETTE. 

The SI-MKB is the standard muscle research system, which can be configured to study skinned and intact muscle fibers, muscle strips and small whole muscles. The modular design of the SI-MKB allows it to be configured for turnkey solutions for specific applications. The system is built on a solid platform making precise mechanical and optical measurements easy. Like the Cell Tester and the SI-MT systems, the SI-MKB uses SI-KG optical force transducers and is constructed with corrosion-free materials (stainless steel, anodized aluminum or plastic).

Can be configured with specific components, like a linear motor or a stimulator, for measuring:

• Responses to electrical stimulation, including tetany
• Mechanical properties of contracting and relaxing muscle strips
• Isotonic force and the effects of constant load
• Twitch amplitude and kinetics, like contraction and relaxation times and velocities
• Slack, quick stretch-release, constant velocity and eccentric contraction tests
• Afterloaded contractions from cardiac muscles
• Vibrational studies that simulate unloaded muscle shortening
• Quick temperature-change effects on skinned and intact muscle fibers
• Can be equipped with components, like a laser diode or a photometer, for measuring:
  • Sarcomere length/spacing by laser diffraction at the same time muscle force is measured
  • Intracellular calcium concentration/distribution in intact muscle fibers as muscle force is measured
  • ATPase activity in skinned muscle fibers as muscle force is measured
• Supplied with a Labtrax 8/16 data acquisition system and MDAC software for:
  • Recording signals from the force transducer and the motor position monitor, through four analog inputs
  • Controlling the stimulator, or an external stimulus isolator, through an analog output
  • Controlling the position of the linear motor through a second analog output
  • Controlling other devices through two digital outputs

This system includes:

• Base  plate, transducer holder
• Force transducer (choose SI-KG2, SI-KG2A, SI-KG4 or SI-KG4A)
• Motor holder, linear motor (SI-MOTTEST)
• Cuvette table, cuvette
• Oxygenation system
• Evacuation system (SI-VAC)
• Signal conditioning system with transducer amplifier and temperature control module, anti-oscillation module and linear motor amplifier
• Lab-Trax 8/16 data acquisition system with MDAC software for recording force and position signals and for  controlling stimulation and the movement of the linear motor.
• Set of 2 tissue mounting supports

*The total price of the system varies, depending on the options selected. Please call to talk with a staff physiologist who can help configure your system to meet your needs.

SI-MT Muscle Tester/MKB Electonics Instruction Manual

ATPase Measurements

Allen, K., Xu, Y. Y., and Kerrick G. W.: Ca2+ measurements in skinned cardiac fibres: effects of Mg2+ on Ca2+ activation of force and fibre ATPase. J. Appl. Physiol. 88:180-185, 2000

Bishow, B., Adhikari, and Wang, K.,: S100A1 modulates skeletal muscle contraction by desensitizing calciuzm activation of isometric tension, stiffness and ATPase. FEBS Letters 497 (2001) 95-98

Brixius, K., Schwinger, R. H. G.: Modulation of cross-bridge interaction by 2,3-butanedoine monoxime in human ventricular myocardium. Naunyn-Schmiedeberg`s Arch. Pharmacol. (2000) 361:440-444

Brixius, K., Mehlhorn, U., Bloch,W., and Schwinger, R. H. G.:Different Effect of the Ca2+ Sensitizers EMD 57033 and CGP 48506 on Cross-Bridge Cycling in Human Myocardium. The Journal of Pharmacology and Experimental Therapeutics JPET 295:1284-1290,2000

Galler, S., Hilber, K., and Göbesberger, A.: Effects of nitric oxide on force-generating proteins of skeletal muscle. Pflügers Arch.-Eur. J. Physiol. 434:242-245 (1997)

Schwinger, R. H. G., Brixius, K., Savvidou-Zaroti, P., Bölck, B., Zobel, C., Frank, K., Kranias, E.G., Hoischen, S., and Erdmann, E.: The enhanced contractility in phospholamban deficient mouse hearts is not associated with alterations in Ca2+-sensitivity öplor myosin ATPase-activity of the contractile proteins. Basic Res. Cardiol. 95:12-18 (2000)

Wang, Y., Xu, Y., Guth, K., and Kerrick, G. W.: Troponin C regulates the rate constant for the dissociation of force-generating myosin cross-bridges in cardiac muscle. Journal of muscle Research and Cell Motility 20:645-653, 1999

Mechanical Experiments

Bonz, A., Laser, M., Küllmer, S., Kniesch, S., Babin-Ebel., Popp, V., Ertl, G., Wagner, JA: Cannabinoids acting on CB1 receptors decrease contractile performance in human atrial muscle. J Cardiovasc Pharmacol. 2003; 41(4):657-664

Bonz, A., Kniesch, S., Hofmann, U., Küllmer, S., Bauer, L., Wagner, L., Ertl, G., and Spindler, M.: Functional properties and [Ca2+ i] metabolism of creatine kinase-KO mice myocardium Biochem. Biophys. Res. Commun. 2002; 298 (1):163-8

Bonz, A., Vahl, C.F., Timek, T., and Hagl, S.: Contractile mapping of human recipient hearts. In M.M. Körfer, H Posival, R. Körfer (eds): Thoracic organ transplantation 1994; 1: 323 – 333

Bonz, A., Herold, U., Vahl, C.F., and Hagl S: Doxorubicin induced cardiomyopathy: comparison of physiological and morphological analysis. Cor. Europaeum 1994; 3 (1): 27-33

Herzig, J.W., Depersin, H. and Leijendekker, W.J.: Mycordial Calcium Sensitivity and Energy Turnover as Modulated by Inorganic Phosphate and EMD 53998, a Novel Inotropic Agent. In: New Aspect in Treatment of Failing Heart, Eds.: Yasuda, H. and Morgan,H.E.,SpringerTokyo,1992,pp.128-132.

Hilber, K., and Galler, S.: Effects of myofibrillar bundle diameter on the unloaded shortening velocity of skinned skeletal muscle fibres. Journal of Muscle Research and Cell Motility 19:143-155 (1998)

Hofmann, U., Domeier, E., Laser, M., Frantz, S., Weckler, B., Kuhlencordt, P., Wagner, H., Popp, V., Keweloh, B., Ertl, G., and Bonz, A.: Impaired myocardial economy of tumor necrosis factor alpha (TNFα) is related to a sphingosine signalling pathway. Am. J. Physiol. Heart Circ. Physiol. 2003;284(6): H2100-H2105

Holubarsch, C., Lüdemann, J., Wiessner, S., Ruf, T., Schulte-Bauklof, H., Schmidt-Schweda, S., Pieske, B., Posival, H., and Just, H.: Shortening versus isometric contractionin isolated human failingand non-failing left ventricular myocardium:dependency of external workand force on muscle length, heart rate and inotropic stimulation. Cardiovasc. Res. 1998; 37:46-57

Isac, C., M., Ruiz, P., Pfitzmaier, H., Birchmaier, W., and Morano, I.: Plakoglobin is Essential for Myocardial Compliance but Dispensable for Myofibril Insertion into Adherens Junctions. Journal of Cellular Biochemistry 72:8-15 (1999)

Kayhan, N., Schmidt, T.M., Bonz, A., Sonnenberg, K., Vahl, C.F., and Hagl, S.: Effects of trifluoperazine on the contraction kinetics of the isolated intact tracheal and pulmonary artery smooth muscle. Thorac Cardiovasc. Surg. 2002; 50(1): 49-54

Leijendekker, W.J. and Herzig, J.W.: Reduction of Myocardial Cross-Bridge Turnover Rate in Presence of EMD 53998, a Novel Ca Sensitizing Agent. Pfluger's Arch. Eur. J. Physiol. 421, 388-390, 1992.

Morano, M, Zacharzowsky, U., Maier, M., Lange P., E., Alexi-Meskishvili, V., Haase, H., and Morano, I.: Regulation of Human Heart Contractility by Essential Myosin Light Chain Isoforms. J. Clin. Invest. 98:2, 467-473 (1996)

Morano, M., Chai, G., Leonidas, G., Lamounier-Zepter, V., Lutsch, G., Kott, M., Haase, H., and Bader, M.: Smooth-muscle contraction without smooth-muscle myosin. Nature Cell Biology 2: 371-375, June 2000

Morano, I., Ritter, O., Michel, G., Bonz, A., Timek, T., and Vahl, C.F.: Interaction between type 1 myosin light chain and actin modulates force of contraction of human heart fibres. Circ. Res. 76 1995; (5): 720-725

Pieske, B., Trost, S., Schütt, K., Minami, K., Just, H., and Hasenfuss, G.: Influence of Forskolinon the force-frequency behavior in nonfailing and end-stage failing human myocardium. Basic Res. Cardiol. 1998; 93: 66-75

Rosenberg, M., Schäffer, L., Bonz, A., Vahl, C.F., and Hagl, S.: Die negativ inotrope Wirkung von Interleukin-1 und Interleukin-6 wird beim Menschen nicht auf der Ebene des kontraktilen Apparates vermittelt. Z Herz-, Thorax- und Gefäßchir. 2000; 14: 155-162

Timek, T., Vahl, C.F., Bonz, A., Schäffer, L., Rosenberg, M., and Hagl, S.: Triiodothyronine reverses depressed contractile performance after excessive catecholamine stimulation. Ann. Thorac Surg. 1998; 66: 1618-1625

Vahl, C.F., Timek, T., Bonz, A., Kochsiek, N., Schäffer, I., Rosenberg, M., Dillmann, R., and Hagl, S.: Myocardial force-length relationship in end-stage dilated cardiomyopathy and normal myocardium: analysis of intact and skinned left ventricular trabeculae obtained during 11 heart transplantations. Basic Res. Cardiol. 1997; 92: 261-270

Vahl, C.F., Bauernschmitt, R., Bonz, A., Herold, U., Lang, A., Ziegler, S., and Hagl, S.: Increased resistance against shortening in myocardium from recipient hearts of 7 patients transplanted for dilated cardiomyopathy. Thoracic Cardiovasc. Surgeon 1993; 41: 224 - 232

Vahl, C.F., Bauernschmitt, R., Bonz, A., Herold, U., Ziegler, S., Lang, A., and Hagl, S.: Contractile behaviour of skinned papillary muscle in mitral valve disease. Thorac Cardiovasc. Surgeon 1992; 40: 253-260

Vahl, C.F., Bauernschmitt, R., Tischmeyer, K., Sonnenberg, K., Lang, A., Bonz, A., and Hagl, S.: Einsatz der vibrationsvermitteleten "force-clamping" - Technik zur Kontraktilitätsbestimmung demembranisierter isolierter myocardialer Muskelfasern. Herz-, Thorax-, Gefäßchir. 1992; 6 : 323-331

Weisser, J., Martin, J., Bisping, E., Maier, L.S., Hasenfuss, G., and Pieske, B.: Mild hypothermia improves circulatory function. Bas. Res. Cardiol. 2001; 96: 198-205

Intracellular Ca++

Bonz, A., Vahl, C.F., and Hagl, S.: Contractile behaviour and intracellular calcium handling during afterloaded contractions in mitral valve disease. Thoracic and Cardiovasc. Surgeon 1997; 45:280-286

Brixius, K., Pietsch, M., Hoischen, S., Müller-Ehmsen, J., and Schwinger, R. H. G.: Effect of inotropic interventions on contraction and Ca+ transients in the human heart. American Journal Physiol. 1997;83:652-660

Brixius, K., Mohr, V., Müller-Ehmsen, J., Hoischen, S., Münch, G., and Schwinger, R. H. G.: Potent vasodilatory with minor cardiodepressant actions of mibefradil in human cardiac tissue. British Journal of Pharmacology (1998) 125, 41-48

Brixius,K., Pietsch, M., and Schwinger, R. H. G.: The intracellular Ca2+-homeostasis influences the frequency-dependent force-generation in man.Basic Res. Cardiol. 94:152-158 (1999)

Brixius, K.,Reicke, S., and Schwinger, R. H. G.: Beneficial effects of the Ca2+ sensitizer levosimendan in human myocardium. Am. J. Physiol.Heart Circ. Physiol.282:H131-H137, 2002

Hasenfuss, G., Pieske, B., Castell, M., Kretschmann, B., Maier, L. S., and Just, H.: Influence of the novel inotropic agent levosimendanon isometric tension and calcium cyclingin failing human myocardium. Circulation 1998; 98: 2141-2147

Hasenfuss, G., Maier, L.S., Hermann, H.P., Luers, C., Hünlich, M., Zeitz, O., Janssen, P.M.L.,and Pieske, B.: Influence of pyruvate on contractile performance and Ca2+-cycling in isolated failing human myocardium. Circulation 2002; 105: 194-199

Knot, Harm J., Taylor, David G., Parilak, Leonard D., LeWinter, Martin M.: Quantification of the rat left ventricle force and Ca2+-frequency relationships: similarities to dog and human. Cardiovascular Research 2004; 61: 77-86

Maier, L.S., Barckhausen, P., Weisser, J., Aleksic, I., Baryalai, M., and Pieske, B.: Ca2+ -handling in isolated human artrial myocardium. Am. J. Physiol. 2000; 279: H952-H958

Maier, L.S.Bers, D.M., Pieske, B.: Differences in Ca2+-handling and sarcoplamatic reticulum Ca2+-content in isolated rat and rabbit myocardium. J.Mol.Cell Cardiol. 2000; 32: 2249-2258

Von Lewinski, D., Voss, K., Kögler, H., and Pieske, B.: Insulin-like growth factor-1 exerts Ca2+-dependent positive inotropic effects in failing human myocardium. Circ.Res.2003; accepted

Pieske, P., Kretschmann, B., Meyer, M., Holubarsch, Ch., Posival, H., Just, H., and Hasenfuss, G.: Alterations in intracellular calcium handling associated with the inverse force-frequency relationin human dilated cardiomyopathy. Circulation 1995;92:1169-1178

Pieske, B., Sütterlin, M., Schmidt-Schweda, S., Minami, K., Meyer, M., Olschewski, M., Holubarsch, Ch., Just, H., and Hasenfuss, G.: Diminished post-rest potentiation of contractile force in human dilated cardiomyopathy-functional evidence for alterations in intracellular Ca2+ handling. J. Clin. Invest 1996;98:764-776

Pieske, B., Schlotthauer, K., Schattmann, J., Beyersdorf, F., Martin, J., Just, H., and Hasenfuss, G.: Ca+-dependent and Ca2+-independent regulation of contractility in isolated human myocardium. Basic Res. Cardiol. 1997; 92 (Suppl.I): 75-86

Pieske, B., Maier, L.S., Bers, D.M., and Hasenfuss, G.: Ca2+handling and SRCa2+ content in isolated failing and nonfailing myocardium.Circ.Res. 1999; 85:38-46

Pieske, B., Maier, L.S., Weisser, J., Piacentino, V., Hasenfuss, G., and Houser, S.: Rate dependence of [ Na+]i and contractility in nonfailing and failing human myocardium. Circulation 2002; 106: 447-453

Schäffer, L., Rosenberg, M., Bonz, A., Vahl, C.F., and Hagl, S.: Kontraktionsverhalten und intrazelluläre Calciumtransienten bei ischämischer Kardiomyopathie: der Einfluss der Vorlast, der Nachlast und der Frequenz. Z Herz-, Thorax- und Gefäßchirurgie 1999; 13: 255-266

Schlotthaur, K., Schattmann, J., Bers, D.M., Maier, L.S., Schütt, U., Minami, K.,m Just, H., Hasenfuss, G., and Pieske, B.: Frequency-dependent changes in contribution of SR Ca2+ to Ca2+ transients in failing human myocardium assessed with Ryanodine. J.Mol.Cardiol. 1998; 30: 1285-1294

Schwinger, R. H. G., Brixius, K., Bavendiek, U., Hoischen, S., Muller-Ehmsen, J., Bölck, B., and Erdmann, E. : Effect of Cyclopiazonic Acid on the Force Frequency Relationship in Human Nonfailing Myocardium. The Journal of Pharmacology and Experimental Therapeutics JPET 283:286-292

Timek, T., Bonz, A., Dillmann, R., Vahl, C.F., and Hagl, S.: The effect of Triiodothyronine on calcium sensitivity on the contractile apparatus following epinephrine exposure: implications for donor heart management. J. Heart Lung Transplant. 1998; 17: 931-940

Vahl, C.F., Bonz, A., Hagl, C., Timek, T., Fuchs, H., Kochsiek, N., and Hagl, S.: Cardioplegia on the contracile apparatus level: Evaluation of a new concept for myocardial preservation in pefused pig hearts. Thorac Cardiovasc. Surgeon 1995; 43; 185-193

Vahl, C.F., Bonz, A., ans Hagl, S.: Intracellular calcium transients of working human myocardium of 7 patients transplanted for congestive heart failure. Circ. Res. 1994; 74: 952-958

Vahl, C.F., Bonz, A., Hagl, C., and Hagl, S.: Reversible desensitivation of the contractile apparatus for calcium: a new concept to improve tolerance to cold ischemia? Eur. J. Cardiothorac Surg. 1994; 8: 370-378

Vahl, C.F., Bonz, A., Ziegler, S., Hagl, C., Herold, U., Menge, F., and Hagl, S.: Brain death and myocardial contractility: respnsiveness of the contractile apparatus for calcium in human hearts after epinephrine stimulation In M.M. Körfer, H Posival, R. Körfer (eds): Thoracic organ transplantation 1994; 1: 15 - 23

Vahl, C.F., Bonz, A., and Hagl, S.: Modulation of the intracellular calcium transient by shortening in normal human atrial and ventricular myocardium from donor hearts. Cor Europaeum 1992; 4 : 130-136

Vahl, C.F., Elkin, M., Jung, A., Schäffer, L., Bonz, A., Castell, M., Albers, J., De Simone, R., and Hagl, S.: Intrazelluläre Calciumtransienten bei nachbelasteten Kontraktionen: Untersuchungen am linksventrikulären Myokard von Patienten mit Aortenklappenstenosen. Z. Kardiovask. Med. 1997; 1: 66-75

Vahl, C.F., Timek, T., Bonz, A., Fuchs, H., Dillmann, R., and Hagl, S.: Length dependence of calcium- and force-transients in normal and failing human myocardium J. Mol. Cell Cardiol. 1998; 30(5): 957-66

Wang, Y., Xu, Y., Guth, K., and Kerrick, G.W.: Troponin C regulates the rate constant for the dissociation of fore-generating myosin cross-bridges in cardiac muscle. Journal of Muscle Research and Cell Motility 20: 645-653 (1999)

To place an order for this product, please contact the appropriate WPI office or distributor.

Customers from North America can order direct from our corporate office:
World Precision Instruments
175 Sarasota Center Boulevard
Sarasota, FL 34240-9258

• Phone or Fax
  Toll-free: 866-606-1974 Tel: 941-371-1003 Fax: 941-377-5428
Our Customer Service Representatives are available 8am - 6pm EST.
• Online Store
  Many of our products are available for purchase through our online store. At this time we can only accept orders from the U.S. through the online store.
• Want instant pricing? Order a free catalog here

International customers, please see our office and distributor contact information list.

Additional Components

Lab-Trax4-24T	A-D Recorder (Requires SI-MACP)
SI-MACP	Linear Motor Action Control Panel
SI-DAS	SI-H Data Recorder
A385R	Stimulator
SI-FS	Field Stimulation Electrodes

No application notes are available for this product at this time.