Nitric Oxide Sensor - 2mm

The image (right) shows an abdominal x-ray of an apparatus consisting of two customized ISO-NOP nitric oxide probes, 4-channel pH catheter and Teflon nasogastic tube. (Courtesy Prof. K.E.L. McColl, University Department of Medicine and Therapeutics, Western Infirmary, Glasgow, Scotland., lijima, K., et al. Gastroenterology 2003:122:1248-1257)

The original nitric oxide probe

Ideal for cell cultures, cell suspensions and many other applications.The ISO-NOP is a popular, robust and high performance sensor encased within a 2 mm diameter disposable stainless steel protective sleeve. The tip of the sleeve is covered with a NO-selective membrane. Replacement membrane sleeves can be purchased separately (WPI #5436) and require an internal electrolyte (WPI #7325).

NO_‾3 and NO_‾2 Detection

A simple change in experimental protocol will enable the ISO-NOP to be conveniently used for rapid accurate determination of nitrite (NO‾₂) and nitrate (NO‾₃) concentration in samples. Using this method a detection limit for NO_‾2 or NO‾₃ as low as 1nM is routinely possible.

Selectivity of WPI's NO sensors

The ideal NO sensor should be insensitive to other reactive species likely to be present within the measurement environment. Conventional Nafion coated carbon fiber NO sensor exhibits a large response to such species. WPI's unique NO sensor technology utilizes an novel surface membrane which amplifies the response to NO whilst eliminating responses to a vast range of reactive species, including nitrite, absorbic acid, hydrogen peroxide, catecolamines, and much more.

Outside Diameter	2mm
Response Time	<5 sec
Lowest Detection Limit/Range	1nM
Upper Detection Limit/Range	80uM (beyond 80uM, sensor accuracy is not guaranteed)
Nominal Sensitivity (New sensor)	≤2pA/nM
Interferent (Selectivity Coefficient)	NaNO2 (10-6 or better)
Drift	<1pA/min.
Poise Voltage	865mV
Typical Quiescent Baseline Current, 25°C	3,000pA
Acceptable Baseline Range	1,000-8,000pA
Polarization Time	12+ hours
Recommended Polarization Solution	0.1M KI/H2SO4
Physiological Interference	None

ISO-NOP Instruction Manual with Calibration Procedure

J.E. Doeller, T. S. Isbell, G. Benavides, J. Koenitzer, H.Patel, R. P. Patel, J. R. Lancaster, Jr. V. M. Darley-Usmar, D.W. Kraus "Polarogaphic measurement of hydrogen sulfide production and consumption by mammalian tissues" Analytical Biochemistry 341. 2005: 40-51

To place an order for this product, please contact the appropriate WPI office or distributor.

Customers from North America can order direct from our corporate office:
World Precision Instruments
175 Sarasota Center Boulevard
Sarasota, FL 34240-9258

• Phone or Fax
  Toll-free: 866-606-1974 Tel: 941-371-1003 Fax: 941-377-5428
Our Customer Service Representatives are available 8am - 6pm EST.
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International customers, please see our office and distributor contact information list.

5399	T-Adapter Kit (pkg. of 3)
5435	ISO-NOP Startup Kit
5436	Replacement Sleeves (pkg. of 4)
7325	ISO-NO Electrolyte Filling Solution (10mL)
7357	Nitrite Standard Solution, 1g/L (100mL)
7363-4	Enzol - Enzymatic detergent (1 gal.)
7364	Cidex
7521	ISONO Electrolyte, CO2 insensitive Filling Solution (10mL)
JUV	ISO-NOP Rejuvenator
MF28G67-5	MicroFil electrolyte filling needle (pkg. of 5)
NSA-3	Pre-Polarizer – 3 channel, ISO-NO Activator

 

No application notes are available for this product at this time.