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Fluorodish Cell Culture Dish - 35mm

Order # FD35COL-100

Fluorodish Cell Culture Dish - 35mm
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  • Optical quality glass bottom for better imaging quality
  • Low sample volume for expensive chemicals
  • Lowest access angle for micropipette
  • Low toxicity adhesive for embryo research



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Measurementsfluorodish.jpg

ID: 33mm * OD: 35.5mm * Glass Diameter Φ: 23.5mm * height (inside): 7.8mm * Height (outside): 9mm * Access angle: 29 degrees

Exceptional Imaging Quality

WPI’s FluoroDish™ tissue culture dishes are now available in a larger range of sizes and coatings. These polycarbonate dishes provide exceptional imaging quality for many applications requiring the use of inverted microscopes such as high resolution image analysis, microinjection and electrophysical recording of fluorescent-tagged cells. Taking advantage of WPI’s extensive experience with low toxicity adhesives, FluoroDish uses a specially formulated adhesive that is optically clear, durable and with extremely low toxicity. Tests by an independent laboratory have shown that the 96-hour surviving rate of embryos is 100% when kept in FluoroDish, substantially better than some other brands. The bottom glass has superior UV transmission (30% transmission at 300 nm, compared to less than 7% for the most popular German glass). Stringent quality control ensures that glass thickness stays within the 0.17 ±0.01 mm range.

Excellent for both classical and fluorescence microscopy

Each WPI dish has a flat (0.17 mm thick) optical quality glass bottom, allowing the use of a short objective working distance, large numerical aperture (NA), and a high magnification (up to 100x). The larger NA and higher magnification provide superior quality imaging for both classical and fluorescence microscopy. Higher effective NA yields brighter images for fluorescence and higher resolution in Image Analysis. The glass bottom permits the use of immersion objectives with medium such as water, glycerin or oil for the highest magnification possible. To optimize heat-exchange, WPI’s glass-bottom dish is designed to be flush (flat) with the microscope stage or heating unit, therefore eliminating the air gap that exists with modified plastic dishes in which a glass cover slip has been inserted.

Materials

An inner well is created within the dish by the glass bottom and the tissue culture grade polystyrene which forms the sides of the dish. All WPI dishes have the advantages of low toxicity and good UV transmission bottom glass. They are individually packed and gamma sterilized.

Features

The 35 mm dish has outside dimensions similar to that of a Corning 35 mm dish and has ø23.5 mm glass window (FD35). Most heaters and perfusion adapters designed for the Corning 35 mm dish will also fit this dish. The type I rat tail collagen used in the coating of this dish has been reported to improve the adhesion of muscle cells.

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To place an order for this product, please contact the appropriate WPI office or distributor.

Customers from North America can order direct from our corporate office:
World Precision Instruments
175 Sarasota Center Boulevard
Sarasota, FL 34240-9258
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    Toll-free: 866-606-1974 Tel: 941-371-1003 Fax: 941-377-5428
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    Many of our products are available for purchase through our online store. At this time we can only accept orders from the U.S. through the online store.
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International customers, please see our office and distributor contact information list.
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No application notes are available for this product at this time.
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Compound Microscopes

Used for higher magnification applications on very small objects from 1 mm to 5 um. These microscopes can reach magnifications from 40X up to 1000X.



Inverted Microscopes

Used in applications such as low to medium magnifications (50X to 200X) such as in patch clamping and large cell injections where the object is in a small liquid bath with circulation and the illumination comes from above the sample. WPI offers two styles of inverted microscopes, one with a movable stage and the other a fixed stage. Both styles have phase contrast image enhancement options for viewing the details of transparent cells.



Stereo Microscopes

Used in lower magnification applications on larger objects up to 50X. Typical usage in small animal desktop surgery and viewing macro objects up to a few centimeters in size.



Surgical Microscopes

Used in animal surgery in a stand up configuration. Typically these use very high definition optics at a lower magnification



Cameras

WPI offers a variety of microscope camera options for image recording, measurement and analysis. The selection of the camera may be mounted on a trinocular (a third optical port specifically designed for camera mounts) or may replace an microscope ocular. To compare the three digital cameras for microscopes, see the Camera Comparison Chart.



Light Sources

Used to illuminate the subject under stereo microscopes and other applications where an external light source is required.



Microscopy Accessories

Accessories for microscopy include spare objectives, eyepieces, C-mount attachments, lamps.



Last Updated: Friday, 10 February 2012 07:46


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