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Set of 11 Calcium Buffers

Order # CALBUF-2

Set of 11 Calcium Buffers
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  • Use with calcium florescent indicators
  • 20 mL of each buffer



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Details

CALBUF-2 is especially suitable for calibrating fluorescent Ca++ indicators. It provides eleven buffer standards in the 10-4 to 10-8 M Ca++ range, where­as other commonly used fluorescent Ca++ indicators have the apparent Kd in the range of 100 to 300 nM. As with any ionic sensitive indicator, the sensitivity range of these indicators is about 1.0 log unit above and below the Kd. CALBUF-2 provides seven calibration points in this sensitivity range. It has an osmolarity of 0.305, which is isotonic with most mammalian cells.

Shelf life

Limited shelf life; use within 30 days.

Concentrations

1x10-8, 4x10-8, 1x10-7, 2.5x10-7, 5x10-7, 7.5x10-7, 1x10-6, 4x10-6, 1x10-5, 4x10-5, and 1x10-4 M at 20°C. Ionic strength: 0.150?M. 11 bottles, 20 mL each.

There are no specifications for this product.
D.D. McKemy, W. Welch, J.A. Airey, J.L.Sutko "Concentrationsof caffeine greater than 20 mM increase the indo-1 fluorescence ratio in a Ca2+- independent manner" Annals of Botany 86. 2000: 687-703
To place an order for this product, please contact the appropriate WPI office or distributor.

Customers from North America can order direct from our corporate office:
World Precision Instruments
175 Sarasota Center Boulevard
Sarasota, FL 34240-9258
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No application notes are available for this product at this time.
Last Updated: Friday, 25 May 2012 01:04


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